After reconstitution, this product may be stored for a maximum of two weeks at 2–8 °C or stored as aliquots at -20 °C. Prolonged storage and repeated freezing and thawing of the reconstituted product are not recommended. Storage at -80 °C has not been tested.
E1257
Epidermal Growth Factor from murine submaxillary gland
lyophilized powder, BioReagent, suitable for cell culture
Synonym(s):
EGF, Epidermal cell growth factor
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| Size/SKU | Availability | Price |
|---|---|---|
0.1 mg | Please contact Customer Service for Availability | 10 500,00 CZK |
About This Item
10 500,00 CZK
Please contact Customer Service for Availability
biological source
mouse submaxillary glands
Quality Segment
product line
BioReagent
assay
≥98% (SDS-PAGE)
form
lyophilized powder
potency
0.02-2.0 ng/mL EC50
packaging
pkg of 0.1 mg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
white
UniProt accession no.
storage temp.
2-8°C
Gene Information
mouse ... Egf(13645)
General description
Epidermal growth factor (EGF) is the member of EGF-like family of growth factors, that binds to EGF receptor. It is majorly expressed in kidney, parotid gland and salivary gland.
Application
Epidermal Growth Factor from murine submaxillary gland has been used
- in studying its effects on the development of caustic esophageal stricture in rats
- for preparation of MCF-10A cell line
- for the culture of primary hair follicle stem cells from arbas cashmere goat (gHFSCs) and adhesion of hair follicles (HF)
Biochem/physiol Actions
Epidermal growth factor (EGF) blocks gastric acid and pepsin secretion and increases gastrointestinal cell proliferation. EGF stimulates proliferation and migration in bone marrow stromal cells (BMSC), that gives rise to osteoblasts, chondrocytes and adipocytes. It functions as a strong mitogenic factor for various cultured cells. Mutations in this gene is linked to hypomagnesemia. EGF plays a major role in the maintenance of hair and skin development.
Physical form
Lyophilized from 0.2 μm filtered solution in ammonium acetate buffer.
Preparation Note
Purified by gel filtration and ion-exchange chromatography.
Analysis Note
The biological activity is measured in a proliferation assay using BALB/MK cells.
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This Item | |||
|---|---|---|---|
| description lyophilized powder, BioReagent, suitable for cell culture | description EGF | description NGF-7S, lyophilized powder, suitable for cell culture | description NGF-2.5S, lyophilized powder, suitable for cell culture |
| biological source mouse submaxillary glands | biological source mouse | biological source mouse | biological source mouse |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| assay ≥98% (SDS-PAGE) | assay ≥90% (SDS-PAGE) | assay - | assay - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| impurities endotoxin, tested | impurities ≤1 EU/μg endotoxin (Protein) | impurities ≤10 | impurities - |
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Storage Class
11 - Combustible Solids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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If dissolved into solution, how long is this product good for when stored at -80C?
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