E1908

Anti-ELMO (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-CED12, Anti-ELMO1, Anti-Engulfment and cell motility 1
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~75 kDa
• species reactivity: rat, mouse, human
• technique(s): immunoprecipitation (IP): 1-2 μL using whole extract of mouse A-20 cells., western blot: 1:1,000-1:2,000 using whole extract of rat brain, western blot: 1:2,000-1:3,000 using whole extract of human Jurkat cells.
• UniProt accession no.: Q92556
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... ELMO1(9844) ; mouse ... Elmo1(140580) ; rat ... Elmo1(361251)

Description

General description

ELMO1 (engulfment and cell motility 1) is a member of the evolutionarily conserved family of ELMO proteins.

Application

Anti-ELMO antibody produced in rabbit is suitable for immunoprecipitation at a working antibody amount of 1-2μL using a whole extract of mouse A-20 cells. It is also suitable for immunoblotting at a working dilution of 1:2000-1:3000 using a whole extract of human Jurkat cells and at 1:1000-1:2000 using a whole extract of rat brain.

Biochem/physiol Actions

ELMO proteins regulate actin cytoskeleton reorganization during engulfment and cell migration. ELMO1 and Dock180 function together as a guanine exchange factor (GEF) for the small GTPase Rac, leading to its activation and cytoskeleton rearrangement. The C-terminal PH domain of ELMO1 binds to Dock180 and the N-terminal binds to the active form of the small GTPase RhoG, targeting the ELMO/Dock180 complex to the membrane. It also interacts with BAI1, an engulfment receptor for apoptotic cells upstream of the ELMO1/Dock180/Rac module. Increased expression of ELMO1 and Dock180 are associated with the invasive phenotype of glioma cells.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)