E3403

EZview^™ Red Protein G Affinity Gel

• Synonym(s): EZview Affinity Gel, Protein G Affinity Gel
• UNSPSC Code: 12352202
• NACRES: NA.56
• MDL number: MFCD03456743
• Form: liquid

Properties

• form: liquid
• Quality Segment: 200
• analyte chemical class(es): proteins (Immunoglobulins of various mammalian species)
• technique(s): affinity chromatography: suitable, antibody protein purification: suitable, immunoprecipitation (IP): suitable
• capacity: ≥8 mg/mL, gel binding capacity (rabbit IgG)
• storage temp.: −20°C

Description

General description

EZ view Red Protein G Affinity Gel is Protein G covalently bonded to 4% Agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue, for purification of proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results.

Application

When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview^™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Features and Benefits

• Increased visibility - Red color reduces risk of incidental aspiration
• Improved recovery of target protein by reduced accidental loss
• Higher reproducibility - More consistent yields

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and MIT-HClis present at 30 PPM and 0.003% (w/v).

50% slurry in PBS with 50%Glycerol and 0.0015%

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable