F8646

Anti-Mouse IgG (Fc specific) F(ab′)₂ fragment−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162628
• Conjugate: FITC conjugate
• Clone: polyclonal
• Application: IHC (p)
• Citations: 6

Properties

• biological source: goat
• Quality Segment: 200
• conjugate: FITC conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• storage condition: protect from light
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (Fc specific) F(ab′)₂ fragment−FITC antibody is specific for the Fc fragment of mouse IgG. No cross reactivity is observed with the Fab fragment of mouse IgG. Purified antibody is conjugated to fluorescein isothiocyanate (FITC) in an alkaline reaction and then purified to remove unbound FITC.

Immunogen

Purified mouse IgG

Application

Anti-Mouse IgG (Fc specific) F(ab′)₂ fragment−FITC antibody may be used for immunohistochemistry at a working dilution of 1:320 using formalin-fixed, paraffin-embedded sections of human tonsil. Antibody dilution of 1:200 was used to label sections of human brainstems and cervical spinal cords with primary antibody against human Bcl-2 protein. The antibody was also used to label erythrocytes for photon reassignment microscopy and for FACS using melanoma cells.

Biochem/physiol Actions

Binds mouse IgG; does not bind other mouse Igs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background with bovine, equine, or human samples.
Useful when trying to avoid background staining due to the presence of Fc receptors.

Other Notes

Antibody adsorbed with bovine, equine, and human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): dust mask type N95 (US), Eyeshields, Gloves