F9259

Anti-Mouse IgM (μ-chain specific)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Mouse IgM antibody
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162659
• Conjugate: FITC conjugate
• Clone: polyclonal
• Application: IF (d)
• Citations: 34

Properties

• biological source: goat
• Quality Segment: 200
• conjugate: FITC conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• technique(s): direct immunofluorescence: 1:128
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

IgM antibodies are present as pentamers in the serum and are produced in response to antigens
Anti-Mouse IgM (μ-chain specific)-FITC antibody is specific for mouse IgM with mu chain. The antibody preparation is specific for mouse IgM when tested against purified mouse IgA, IgG (all subclasses), and IgM. Goat anti-mouse IgM is then conjugated to Fluorescein Isothiocyanate (FITC), Isomer I.

Immunogen

Purified mouse IgM

Application

Anti-Mouse IgM (μ-chain specific)-FITC antibody may be used for immunofluorescent labeling of mouse spleen cells at a minimum working antibody dilution of 1:128. It was also used for immunohistochemistry of rat heart tissue sections at a working dilution of 1:50. A dilution of 1:100 was used to label human ovarian-adenocarcinoma cells for immunofluorescence.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

Binds mouse IgM only; does not bind other mouse Igs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable