This product is supplied as a 50% glycerol solution containing ~9 mg per mL bovine serum albumin, 30% sucrose, 1.5 mM EDTA, 1.5 mM EGTA, 1.5 mM 2-mercaptoethanol, 0.3% TRITON™ X-100, 0.003% sodium azide, and 15 mM potassium phosphate, pH 6.8. For assay purposes, the product is diluted in 50 mM MOP HCl Buffer, pH 7.4. Other published literature describes dilution to 1 U/mL using 20 mM sodium phosphate buffer containing 1 mM magnesium chloride (pH 7.2).
K1502
α-Ketoglutarate Dehydrogenase from porcine heart
buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry)
Synonym(s):
Multienzyme 2-oxoglutarate dehydrogenase complex
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| Size/SKU | Availability | Price |
|---|---|---|
20 units | Please contact Customer Service for Availability | 6 080,00 CZK |
50 units | Please contact Customer Service for Availability | 12 600,00 CZK |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
0.1-1.0 units/mg protein (Lowry)
form
buffered aqueous glycerol solution
Quality Segment
specific activity
0.1-1.0 units/mg protein (Lowry)
foreign activity
pyruvate dehydrogenase ≤20%
shipped in
dry ice
storage temp.
−20°C
General description
Research Area: Neuroscience
α-Ketoglutarate dehydrogenase (α-KGDH) is a multienzyme complex localized to the mitochondria. This integrated enzyme is made up of many units of thiamine pyrophosphate-dependent dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and dihydrolipoamide succinyl transferase (E2).
α-Ketoglutarate dehydrogenase (α-KGDH) is a multienzyme complex localized to the mitochondria. This integrated enzyme is made up of many units of thiamine pyrophosphate-dependent dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and dihydrolipoamide succinyl transferase (E2).
Application
α-Ketoglutarate Dehydrogenase from the porcine heart has been used:
- to study the reversal of nitration by glutathione (GSH) in peroxynitrite-treated cells
- to measure its activity by Spectramax M5 microplate spectrofluorimeter using heart mitochondria
- as a positive control to evaluate its activity in by Spectramax GEMINI EM fluorescence microplate reader using mice neurons
Biochem/physiol Actions
α-Ketoglutarate dehydrogenase (α-KGDH) is a key enzyme of bioenergetic processes and a controlling unit of metabolic flux through the Krebs cycle or tricarboxylic acid (TCA) cycle. It catalyzes the oxidative decarboxylation of α-ketoglutarate (KG) to succinyl-CoA by releasing reduced nicotinamide adenine dinucleotide (NADH). It is the rate-limiting reaction of the TCA cycle. This reaction contributes to the electrons of the respiratory chain and requires thiamine pyrophosphate as a cofactor. The reduction of NAD (nicotinamide adenine dinucleotide) is observed to determine its reaction rate. α-KGDH from porcine has an optimum pH range of 6.6–7.4. This enzyme is inhibited by oxidative stress and results in a metabolic deficiency. However, α-KGDH is also known to produce reactive oxygen species (ROS) leading to oxidative stress. Defective or limited levels of α-KGDH cause several neurodegenerative diseases such as Alzheimer′s disease.
α-Ketoglutarate dehydrogenase is most responsive to alterations in the tumor microenvironment and contributes to the adaptive metabolic response in cancer. Inhibiting α-ketoglutarate dehydrogenase counteracts lung metastasis associated with breast cancer.
Physical form
Supplied as a 50% glycerol solution containing ~9 mg per mL bovine serum albumin, 30% sucrose, 1.5 mM EDTA, 1.5 mM EGTA, 1.5 mM 2-mercaptoethanol, 0.3% TRITON™ X-100, 0.003% sodium azide, and 15 mM potassium phosphate, pH 6.8.
Analysis Note
May contain traces of polyethylene glycol.
Other Notes
One unit will convert 1.0 μmole of β-NAD to β-NADH per min at pH 7.4 at 30 °C in the presence of saturating levels of coenzyme A.
Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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This Item | |||
|---|---|---|---|
| description buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry) | description buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry) | description buffered aqueous glycerol solution, ≥600 units/mg protein (biuret) | description Type I, 0.5-3.0 unit/mg solid (plus numerous enzyme activities associated with porcine heart) |
| specific activity 0.1-1.0 units/mg protein (Lowry) | specific activity 0.1-1.0 units/mg protein (Lowry) | specific activity 600-1000 units/mg protein (biuret) | specific activity 0.5-3.0 unit/mg solid (plus numerous enzyme activities associated with porcine heart) |
| form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form solid |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in - |
| foreign activity pyruvate dehydrogenase ≤20% | foreign activity α-ketoglutarate dehydrogenase ≤15% | foreign activity Glutamic-Pyruvic Transaminase ≤0.01%, Glutamic-Oxalacetic Transaminase ≤0.01% | foreign activity - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Storage Class
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
9002-93-1
CAS No.
9002-93-1
CAS No.
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I would like to ask about the recommended solvent or buffer for diluting α-Ketoglutarate dehydrogenase (Product No. K1502). Could you please advise which solvent or buffer is recommended for diluting this enzyme before use?
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