L2011

D-Lactic Dehydrogenase from Lactobacillus leichmannii

ammonium sulfate suspension, ≥250 units/mg protein (biuret)

• Synonym(s): Lactate, (R)-Lactate:NAD⁺ oxidoreductase, D−LDH
• CAS Number: 9028-36-8
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 1.1.1.28 (BRENDA, IUBMB)
• MDL number: MFCD00130907
• Specific activity: ≥250 units/mg protein (biuret)

Properties

• form: ammonium sulfate suspension
• Quality Segment: 200
• specific activity: ≥250 units/mg protein (biuret)
• foreign activity: Malic dehydrogenase <0.5% of base activity
• storage temp.: 2-8°C

Description

General description

Research area: Cell Signalling D-lactate dehydrogenase belongs to the D-isomer-specific 2-hydroxyacid dehydrogenase family. Lactate dehydrogenase (LD) is a tetramer consisting of two (H and M) subunits. It has five isozymes (LD1 to LD5) and this composition varies in different tissues. LD1 is abundant in the kidney, heart, and erythrocytes. LD5 is found in the liver and skeletal muscles. LD is a ubiquitous molecule found in plants, yeast, mammals, and microorganisms and is a member of the oxidoreductase family.

Biochem/physiol Actions

Lactic dehydrogenase (LDH)plays an important role in the glycolytic pathway where it converts pyruvate tolactate by using NAD⁺ as a co-factor. It is considered animportant molecule that can be used in cancer therapy as it acts as aglycolytic inhibitor. Inhibition of LDH is associated with blocking of aerobicglycolysis in tumour cells.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, pH 6.0

Other Notes

One unit will reduce 1.0 μmole of pyruvate to D-lactate per min at pH 7.0 at 25 °C.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)