OGS595

PSF-RENILLA-PHOTINUS - BI-DIRECTIONAL FIREFLY RENILLA LUCIFERASE PLASMID

plasmid vector for molecular cloning

• Synonym(s): cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• NACRES: NA.85
• UNSPSC Code: 12352200
• Origin of replication: pUC (500 copies)
• Bacteria selection: kanamycin
• Reporter gene: Renilla luciferase
• Peptide cleavage: no cleavage

Properties

• form: buffered aqueous solution
• mol wt: size 6170 bp
• bacteria selection: kanamycin
• origin of replication: pUC (500 copies)
• peptide cleavage: no cleavage
• reporter gene: Renilla luciferase
• shipped in: ambient
• storage temp.: −20°C

Description

General description

This plasmid has been designed so that sequences can inserted between two reporter genes (renilla and firefly luciferase) to allow the relative transcription level in both directions to be determined by measuring the reporter luminescence. Dual luciferase reporter assay kits are available from other providers that are compatible with this plasmid. The plasmid only contains a Bgl2 site between the two genes to allow insertion.

Promoter Expression Level:

Application

This plasmid contains two luciferase reporter genes (Firefly and Renilla) in opposing directions with a Bgl2 restriction site in between the two genes to allow promoters to be inserted. This allows the transcriptional level of DNA sequences and promoters to be determined in both directions by inserting the sequences into the Bgl2 restriction site. If the sequence you would like to insert contains a Bgl2 site fragments with BamHI also have the same overhangs and can be ligated into this plasmid cut with Bgl2.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Other Notes

To view sequence information for this product, please visit the product page

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable