P2192

10X PCR Buffer

Optimized for routine PCR with MgCl₂ included

• Synonym(s): PCR Buffer Solution, PCR Reaction Buffer
• NACRES: NA.52
• UNSPSC Code: 41106306

Properties

• Quality Segment: 300
• form: liquid
• packaging: vial of 1.5 mL
• technique(s): PCR: suitable, whole genome amplification: suitable
• color: colorless
• application(s): agriculture
• foreign activity: DNase, RNase, none detected
• storage temp.: −20°C

Description

Application

10X PCR Buffer has been used as a component of the PCR mixture:

• for the amplification of viral DNA
• to amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individuals
• to amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp.

Features and Benefits

• Standalone buffer
• Compatible with JumpStart^™ Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq^® Genomic DNA Polymerase (D8312)

Preparation Note

Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl₂; 0.01% gelatin

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves