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P4591

Pyruvate Oxidase from microorganisms

lyophilized powder, ≥1.5 U/mg

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Synonym(s):

Pyruvate:oxygen oxidoreductase (phosphorylating)

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Size/SKUAvailabilityPrice
100 units
Please contact Customer Service for Availability
7 860,00 CZK

About This Item

CAS Number:
9001-96-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-644-5
MDL number:
MFCD00132148
EC Number:
1.2.3.3 (BRENDA, IUBMB)
Specific activity:
≥1.5 U/mg

7 860,00 CZK

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form

lyophilized powder

Quality Segment

200

specific activity

≥1.5 U/mg

mol wt

~260 kDa

storage temp.

−20°C

General description

Isoelectric point : 4.3
Michaelis constant : 3.4 X 10-4M (Pyruvate)
Inhibitors : Fe++,Zn++,Cu++,Ag+,Hg++
Optimum pH : 5.7
Optimum temperature : 65oC
pH Stability : pH 5.7 - 6.5 (25oC, 20hr)
Thermal stability : below 45oC (pH 6.0, 15min)

Application

Pyruvate Oxidase from microorganisms has been used in the Amplex Red-based fluorescence assay for pyruvate. It has also been used in developing near real-time continuous detection system for pyruvate.
Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and CO2. It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarumand Streptococcus pneumoniae. Pyruvate Oxidase is used for enzymatic determination of pyruvate, GOT, and GPT in clinical analysis.

Biochem/physiol Actions

Pyruvate Oxidase oxidises pyruvate to form acetate, hydrogen peroxide and carbondioxide. Pyruvate oxidase requires flavin adenine dinucleotide (FAD), thiamine pyrophosphate (TPP) and magnesium as cofactors for its catalytic activity. Pyruvate oxidase is activated by the cofactor thiamine.
Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.

Physical form

Lyophilized powder containing FAD and sugar as stabilizer

Other Notes

One unit will produce 1.0 μmole of H2O2 per min during the conversion of pyruvate and phosphate to acetylphosphate and CO2 at pH 5.7 at 37 °C.

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description

lyophilized powder, ≥1.5 U/mg

description

lyophilized powder, ≥35 units/mg protein (biuret)

description

lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli

description

powder, ≥1.5 units/mg solid

specific activity

≥1.5 U/mg

specific activity

≥35 units/mg protein (biuret)

specific activity

≥50 units/mg protein

specific activity

≥1.5 units/mg solid

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

mol wt

~260 kDa

mol wt

-

mol wt

64 kDa

mol wt

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000577258
    0000569107
    0000423691
    0000517864
    0000288360

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