Elemental analysis of this material has not been performed. The sulfate content is not determined.
P8169
Phytagel™
BioReagent, suitable for plant cell culture, powder
Phytagel™
Synonym(s):
Gellan Gum, Agar substitute gelling agent
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100 G
CZK 1,750.00
250 G
CZK 5,050.00
500 G
CZK 8,650.00
1 KG
CZK 16,400.00
5 KG
CZK 43,700.00
About This Item
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product line
BioReagent
form
powder
technique(s)
cell culture | plant: suitable
application(s)
agriculture
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1 of 4
This Item | G1910 | A9799 | A4550 |
|---|---|---|---|
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
| form powder | form - | form powder | form powder |
| technique(s) cell culture | plant: suitable | technique(s) - | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| product line BioReagent | product line - | product line - | product line - |
General description
Typical working concentration: 1.5-2.5 g/L in plant tissue culture media; up to 10 g/L in microbiological media. Phytagel™ requires the presence of cations (especially divalent) for gelling to occur. Concentrations of calcium and magnesium contained in most plant tissue culture media are typically sufficient for gelation. Low-salt media formulations, especially those used in microbiological applications, may require supplementation with additional calcium or magnesium salts (e.g., CaCl2 or MgSO4) or higher concentrations of Phytagel.
Application
Biochem/physiol Actions
Phytagel is intended for use in place of agar and other gelling agent for plant tissue culture. Phytagel has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets.
Preparation Note
To prevent clumping, add slowly to rapidly stirring medium prior to heating.
Other Notes
An agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination.
Legal Information
Phytagel is a trademark of Sigma-Aldrich Co. LLC
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Protocols
Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.
Related Content
Instructions
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What is the sulfate content, if any, of Phytagel?
1 answer-
Helpful?
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Buongiorno. Vorrei sapere se il Phytagel è Gellano ad alto o a basso grado di acilazione
1 answer-
This product is characterized as having a low acyl content.
Helpful?
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What is the molecular weight of one repeating unit?
1 answer-
The molecular weight is not determined. However, the constituent sugars of Phytagel are glucose, glucuronic acid and rhamnose in the molar ratio of 2:1:1.
Helpful?
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What is the gelling strength of Phytagel P8169
1 answer-
The Gel Strength for Phytagel (P8169) is 700 g/cm2 (tested at 0.5%).
Helpful?
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