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PP0200

GlycoProfile™ I, In-Gel Deglycosylation Kit

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Size/SKUAvailabilityPrice
1 kit
Please contact Customer Service for Availability
16 300,00 CZK

About This Item

UNSPSC Code:
12352200
EC Number:
232-615-7
NACRES:
NA.32

16 300,00 CZK

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shelf life

≥1 yr at 2‑8 °C

Quality Segment

200

storage temp.

2-8°C

Application

The GlycoProfile™ Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible method to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.

Biochem/physiol Actions

The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching.

Preparation Note

Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

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GlycoProfile™ I, In-Gel Deglycosylation Kit

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mPAGE®TurboMix Bis-Tris Gel Casting Kit Premixed solutions for casting bis-tris polyacrylamide gels; TMKIT-10 includes 36 mL resolving solution and 20 mL stacking solution; TMKIT-60 includes 216 mL resolving solution and 120 mL stacking solution

Millipore

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Akt Inhibitor VI, Akt-in The Akt Inhibitor VI, Akt-in controls the biological activity of Akt. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

Sigma-Aldrich

124013

Akt Inhibitor VI, Akt-in

description

-

description

sufficient for 20 samples

description

Premixed solutions for casting bis-tris polyacrylamide gels; TMKIT-10 includes 36 mL resolving solution and 20 mL stacking solution; TMKIT-60 includes 216 mL resolving solution and 120 mL stacking solution

description

The Akt Inhibitor VI, Akt-in controls the biological activity of Akt. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shelf life

≥1 yr at 2‑8 °C

shelf life

-

shelf life

-

shelf life

-

Kit Components Only

Product No.
Description
  • Acetonitrile, ≥99.93%, BioSyn® 50 mL

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • P7367
    PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE) 50 U
    SDS
  • T6567
    Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg
    SDS
  • Trypsin Solubilization Reagent
    SDS
  • I0408
    Invertase Glycoprotein Standard, BioReagent, from Saccharomyces cerevisiae
    SDS

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

3 - Flammable liquids

flash_point_f

35.6 °F

flash_point_c

2.0 °C

wgk

WGK 3

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