Unfortunately, published citations on the use of this kit with mouse serum are not available. Please see the image below for an example of expected recovery rates:
RAB0210
Mouse Granzyme B ELISA Kit
for serum, plasma and cell culture supernatant
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Please contact Customer Service for Availability | 14 700,00 CZK |
About This Item
NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
mouse ... Gzmb(14939)
Input:
sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type serum
sample type cell culture supernatant(s)
Species reactivity:
mouse
Storage temp.:
−20°C
Shipped in:
wet ice
14 700,00 CZK
Please contact Customer Service for Availability
species reactivity
mouse
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, capture ELISA: suitable
input
sample type plasma
sample type serum
sample type cell culture supernatant(s)
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 12 pg/mL
standard curve range: 14.34-3500 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... Gzmb(14939)
General description
The Mouse Granzyme B enzyme-linked immunosorbent assay (ELISA) kit is an in vitro assay for the quantitative measurement of mouse granzyme B in serum, plasma, cell culture supernatants and urine. Granzyme B binds to mannose-6-phosphate/insulin-like growth factor II receptor. This protein is implicated in fast DNA fragmentation, a key step towards apoptosis in cells. Granzyme B is involved in the cleaving of pro-caspase-3, which leads to the activation of caspase-3. This protein also cleaves caspase-6, -7, -8, -9, and -10.
The Mouse Granzyme B ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Granzyme B in serum, plasma, cell culture supernatants and urine.
Immunogen
Recombinant Mouse Granzyme B
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
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This Item | |||
|---|---|---|---|
| description for serum, plasma and cell culture supernatant | description for serum, plasma and cell culture supernatants | description for serum, plasma and cell culture supernatants | description recombinant, expressed in Hi-5 Insect cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture |
| species reactivity mouse | species reactivity human | species reactivity human | species reactivity - |
| Gene Information mouse ... Gzmb(14939) | Gene Information human ... GZMH(2999) | Gene Information human ... GZMB(3002) | Gene Information mouse ... GZMB(14939) |
| input sample type plasma | input sample type cell culture supernatant(s) | input sample type cell culture supernatant(s) | input - |
| assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range - |
| technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) cell culture | mammalian: suitable |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method - |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
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It says here that this ELISA kit works for the detection of GzmB in mouse serum, but I can't find any papers that have used serum. Could you please cite a paper or previous work demonstrating the utility of this kit for detecting GzmB in serum?
1 answer
