RAB0216

Human HSP70 ELISA Kit

for serum, plasma, cell culture supernatant and urine

• Synonym(s): HSP70-1, Heat shock 70 kDa protein 1A
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... HSPA4(3308)
• Input: sample type plasma; sample type urine; sample type cell culture supernatant(s); sample type serum
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type plasma; sample type urine; sample type cell culture supernatant(s); sample type serum
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 2 ng/mL; standard curve range: 2.469-600 ng/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... HSPA4(3308)

Description

General description

The Human HSP70 (heat shock protein 70) ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human HSP70 in serum, plasma, cell culture supernatants and urine. HSP70 is an important ATP-dependent cytosolic chaperone along with Hsp90. Hspa4 aids in the folding of several proteins and helps in protecting against cellular stresses such as rise in temperature. HSP70 maintains the function of the mitotic centrosome to coordinates bipolar mitotic spindle assembly. Hspa4 might compensate the loss of parkin function in mice by protecting the cells against proteolytic and mitochondrial stress. Therefore, it can serve as an important therapeutic agent for parkinson disease. Upregulation of HSPA4 is observed in parkin gene knockout mice. Hspa4 is known to be associated with tumor progression and offers resistance against many therapeutic agents. The gene is upregulated in many different types of cancer.

Immunogen

Recombinant Human HSP70

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABELADB

  ELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  SDS
• RABELADC

  ELISA 1X Assay/Sample Diluent Buffer C (Item L)

  SDS
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDS
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDS
• RABWASH4

  20X Wash Buffer (Item B)

  SDS

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Codes: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 10 - Combustible liquids