SML4396

Desthiobiotin-iodoacetamide DbGGI

≥98% (HPLC)

• Synonym(s): DbGGI, Iodoacetamide-desthiobiotin, N-(2-((2-((2-(2-iodoacetamido)ethyl)amino)-2-oxoethyl)amino)-2-oxoethyl)-6-((4R,5S)-5-methyl-2-oxoimidazolidin-4-yl)hexanamide
• Empirical Formula (Hill Notation): C₁₈H₃₁IN₆O₅
• CAS Number: 2907659-77-0
• Molecular Weight: 538.38
• UNSPSC Code: 12352200
• Assay: ≥98% (HPLC)
• Form: powder

Properties

• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: powder
• color: white to pink
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: -10 to -25°C

Description

Biochem/physiol Actions

DbGGI is a bifunctional, cysteine-reactive ABPP probe that links an iodoacetamide warhead to a desthiobiotin tag via a flexible 10-atom chain, providing a "click-free" alternative to alkyne-tagged probes. This design enables strong, reversible capture on streptavidin beads and efficient peptide elution under mild acidic conditions, which eliminates background from copper reagents and yields a cleaner, more accurate representation of pulldown results for mass spectrometry or western blot. The probe′s principal innovation is its application in a quantitative, concentration-dependent enrichment strategy that selectively identifies hyper-reactive primary protein targets (PPTs) in cell lysates—a class often obscured in conventional high-concentration ABPP. As demonstrated in U87MG cell lysates, this approach captured 116 targets at a low concentration (10 µM) versus 193 at a high concentration (250 µM). Note: Differentially label cell lysates (2 mg/mL) with low (10 µM) and high (250 µM) DbGGI from a DMSO stock for 1 hour at 37°C. Then, capture the proteomic targets via a 4-hour incubation with streptavidin beads at room temperature.

Safety Information

• Storage Class: 11 - Combustible Solids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable