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Anti-Mouse IgG (Fab specific)–TRITC antibody produced in goat

Name: Anti-Mouse IgG (Fab specific)–TRITC antibody produced in goat
Brand: SIGMA
Price: 6239 CZK

affinity isolated antibody, buffered aqueous solution

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Size/SKU	Availability	Price
1 mL	Please contact Customer Service for Availability	7 340,00 CZK 6 239,00 CZK

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162624

Conjugate:

TRITC conjugate

Clone:

polyclonal

Application:

IF (d), IHC (p)

Citations:

6 239,00 CZK

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Properties

biological source

goat

Quality Segment

200

conjugate

TRITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:64, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Immunoglobulin G (IgG)antibody is found in four subclasses IgG1, IgG2, IgG3 and IgG4. It has a monomeric structure. IgG is secreted by B cells and is found in blood and extracellular fluids.. Anti-Mouse IgG (Fab specific) is conjugated to tetramethylrhodamine isothiocyanate (TRITC) and then purified by gel filtration to remove free TRITC.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Conjugation of TRITC to Mouse IgG (Fab specific) antibody enables the use in immunofluorescent techniques.

Biochem/physiol Actions

IgG antibody subtype provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Immunoglobulin G (IgG) antibody participates in complement fixation and opsonization.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with bovine, horse or human samples.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	F4143	F5897	A2304
Sigma-Aldrich T7782 Anti-Mouse IgG (Fab specific)–TRITC antibody produced in goat	Sigma-Aldrich F4143 Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat	Sigma-Aldrich F5897 Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat	Sigma-Aldrich A2304 Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution
biological source goat	biological source goat	biological source goat	biological source goat
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate TRITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate	conjugate peroxidase conjugate
technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400, direct immunofluorescence: 1:64	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, indirect immunofluorescence: 1:128	technique(s) indirect immunofluorescence: 1:64, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200	technique(s) western blot: 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells, direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue section
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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