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Cell Uptake and Validation of Novel PECs for Biomedical Applications.

Journal of drug delivery (2011-08-31)
Ilaria E Palamà, Mariarosaria Musarò, Addolorata M L Coluccia, Stefania D'Amone, Giuseppe Gigli
PMID21876815
ABSTRACT

This pilot study provides the proof of principle for biomedical application of novel polyelectrolyte complexes (PECs) obtained via electrostatic interactions between dextran sulphate (DXS) and poly(allylamine hydrochloride) (PAH). Scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed that DXS/PAH polyelectrolyte complexes were Monodispersed with regular rounded-shape features and average diameters of 250 nm at 2 : 1 weight ratios of DXS/PAH. Fluorescently labelled DXS and fluorescein-isothiocyanate- (FITC-)conjugate DXS were used to follow cell uptake efficiency of PECs and biodegradability of their enzymatically degradable DXS-layers by using confocal laser scanning microscopy (CLSM). Moreover, quantitative MTT and Trypan Blue assays were employed to validate PECs as feasible and safe nanoscaled carriers at single-cell level without adverse effects on metabolism and viability.

MATERIALS
Product Number
Brand
Product Description
M5655
Sigma-Aldrich
Thiazolyl Blue Tetrazolium Bromide, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
M2128
Sigma-Aldrich
Thiazolyl Blue Tetrazolium Bromide, 98%
12006C
Sigma-Aldrich
Fetal Bovine Serum, Australia origin, USDA approved, Heat Inactivated, suitable for cell culture
12107C
Sigma-Aldrich
Fetal Bovine Serum, USA origin, γ-irradiated by SER-TAIN process, sterile-filtered, suitable for cell culture
12003C
Sigma-Aldrich
Fetal Bovine Serum, Australia origin, USDA approved, sterile-filtered, suitable for cell culture
12103C
Sigma-Aldrich
Fetal Bovine Serum, USA origin, suitable for cell culture
12106C
Sigma-Aldrich
Fetal Bovine Serum, USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture
12007C
Sigma-Aldrich
Fetal Bovine Serum, Australia origin, γ-irradiated, sterile-filtered, suitable for cell culture
F0392
Sigma-Aldrich
Fetal Bovine Serum, USA origin, Dialyzed by ultrafiltration against 0.15 M NaCl, sterile-filtered, suitable for cell culture
F3297
SAFC
Fetal Bovine Serum, Australia origin, γ-irradiated, suitable for cell culture, suitable for hybridoma
F2442
Sigma-Aldrich
Fetal Bovine Serum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
F9423
Sigma-Aldrich
Fetal Bovine Serum, Australia origin, suitable for, USDA approved, sterile-filtered, suitable for cell culture, suitable for hybridoma
F9665
Sigma-Aldrich
Fetal Bovine Serum, Heat Inactivated, non-USA origin, sterile-filtered, suitable for cell culture
F4135
Sigma-Aldrich
Fetal Bovine Serum, USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma
F6765
Sigma-Aldrich
Fetal Bovine Serum, USA origin, Charcoal Stripped, sterile-filtered, suitable for cell culture
F7524
Sigma-Aldrich
Fetal Bovine Serum, non-USA origin, sterile-filtered, suitable for cell culture
283215
Sigma-Aldrich
Poly(allylamine hydrochloride), average Mw ~17,500 (GPC vs. PEG std.)
283223
Sigma-Aldrich
Poly(allylamine hydrochloride), Mw 40,000-150,000