Skip to Content
Merck
  • [A method for simultaneous assay of propulsion and absorption in small intestine].

[A method for simultaneous assay of propulsion and absorption in small intestine].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences (2012-08-29)
Jian-xiang Liu, Xin-ye Zhang, Dong-mei Pan, Qin-li Zhang, Xiang-rong Li
ABSTRACT

To develop a method for simultaneous assay of propulsion and absorption in small intestine. The mice were administrated through gastric tube with mixed reagents containing 0.12% phenol red, D-xylose (1.25%, 2.5% and 5%) and 15% gelatin. The influence of phenol red on D-xylose absorption and the influence of D-xylose on small intestine propulsion rate were investigated by measuring serum concentration of D-xylose with phloroglucinol method. At 10 min, no significant difference was found between 5% D-xylose mixed reagent group and 5% D-xylose control. At 15 min, small intestine propulsion rate in 5% D-xylose mixed reagent group, but not in 2.5% and 1.25% D-xylose mixed reagent groups, was significantly higher than in phenol red control (P<0.05). Gastric administration of mixed reagent containing 0.12% phenol red, 5% D-xylose and 15% gelatin can simultaneously assay propulsion and absorption of small intestine in mice.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Phenol red solution, 0.5%, liquid, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
Phenol Red, ACS reagent
Sigma-Aldrich
Phenol Red, powder, BioReagent, suitable for cell culture