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  • [The application of three methods combined for extracting DNA from human epithelium].

[The application of three methods combined for extracting DNA from human epithelium].

Fa yi xue za zhi (2009-04-29)
Yan-xia Guo, Song Chen, Kai-hui Liu
ABSTRACT

To improve DNA extraction from human epithelium. Chelex-100, Chelex-100 organic combined and Chelex-100 magnetism pearl combined methods were adopted according to the situation of each case. With the stepwise extracting protocol, the trace amount of DNA was analyzed most efficiently and the success rate of DNA extracting was better improved. The efficiency of DNA extraction from human epithelium could be greatly improved by modifying the extracting protocol such as purification and concentration based on previous results.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chelex® 100 sodium form, 50-100 mesh (dry)
Sigma-Aldrich
Chelex® 100 sodium form, 200-400 mesh particle size
Sigma-Aldrich
Chelex® 100 sodium form, 100-200 mesh particle size