Amino acids and biogenic amines in cerebrospinal fluid of patients with Parkinson's disease.

Amino acids and biogenic amines in cerebrospinal fluid of patients with Parkinson's disease.

Neurochemical research (2003-07-02)

S Engelborghs, B Marescau, P P De Deyn

ABSTRACT

To study changes in amino acid metabolism and biogenic amines in Parkinson's disease, we set up a prospective study and measured biogenic amines, their main metabolites, and 22 different amino acids, in cerebrospinal fluid of Parkinson's disease patients (n = 24) and age-matched controls (n = 30). A trend toward higher dopamine levels in Parkinson's disease patients was interpreted as an effect of treatment with levodopa and/or selegiline. Significantly lower concentrations of the dopamine metabolite 3,4-dihydroxyphenylacetic acid in the Parkinson's disease group might reflect dopaminergic cell loss. Our results revealed decreased serotonin catabolism that was interpreted as an effect of treatment with selegiline. Whereas all amino acid levels were unchanged, taurine was significantly lower in Parkinson's disease patients. Studies showed that taurine exerts a trophic action on the central nervous system. In this view, decreased taurine in a neurodegenerative disorder as Parkinson's disease deserves attention.

MATERIALS

Product Number

Brand

Product Description

398136

Sigma-Aldrich

Ethanolamine, ACS reagent, ≥99.0%

200905

Sigma-Aldrich

Magnesium, turnings, reagent grade, 98%

267074

Sigma-Aldrich

Nickel, rod, diam. 6.35 mm, ≥99.99% trace metals basis

266035

Sigma-Aldrich

Titanium, wire, diam. 1.0 mm, 99.99% trace metals basis

255610

Sigma-Aldrich

Chromium, chips, thickness ~2 mm, 99.5%

366986

Sigma-Aldrich

Molybdenum, powder, 10 μm, ≥99.95% trace metals basis

411000

Sigma-Aldrich

Ethanolamine, purified by redistillation, ≥99.5%

520373

Sigma-Aldrich

Tin, powder, 10 μm, 99% trace metals basis

357626

Sigma-Aldrich

Nickel, wire, diam. 0.5 mm, ≥99.9% trace metals basis

326380

Sigma-Aldrich

Cobalt, foil, thickness 0.1 mm, ≥99.99%

326410

Sigma-Aldrich

Copper, wire, diam. 0.64 mm, 99.995% trace metals basis

326887

Sigma-Aldrich

Aluminum, wire, diam. 0.58 mm, ≥99.99% trace metals basis

266558

Sigma-Aldrich

Aluminum, wire, diam. 1.0 mm, 99.999% trace metals basis

357553

Sigma-Aldrich

Nickel, foil, thickness 0.5 mm, 99.98% trace metals basis

349216

Sigma-Aldrich

Copper, wire, diam. 1.0 mm, ≥99.9%

369489

Sigma-Aldrich

Titanium, foil, thickness 2.0 mm, 99.7% trace metals basis

357588

Sigma-Aldrich

Nickel, foil, thickness 0.1 mm, 99.98% trace metals basis

499579

Sigma-Aldrich

Magnesium preparation, Highly Reactive Rieke^® Metal, suspension, 2.5 g in THF

499552

Sigma-Aldrich

Zinc preparation, 5 g/dL Zn²⁺ in THF, highly reactive Rieke^®metal

349151

Sigma-Aldrich

Copper, foil, thickness 0.5 mm, 99.98% trace metals basis

326429

Sigma-Aldrich

Copper, wire, diam. 0.25 mm, 99.999% trace metals basis

349224

Sigma-Aldrich

Copper, wire, diam. 2.0 mm, 99.999% trace metals basis

266132

Sigma-Aldrich

Manganese, powder, −325 mesh, ≥99% trace metals basis

349208

Sigma-Aldrich

Copper, foil, thickness 0.025 mm, 99.98% trace metals basis

266167

Sigma-Aldrich

Manganese, chips, thickness <2.0 mm, 99%

518573

Sigma-Aldrich

Aluminum, granular, <1 mm, 99.7% trace metals basis

433705

Sigma-Aldrich

Aluminum, evaporation slug, diam. × L 6.3 mm × 6.3 mm, 99.999% trace metals basis

265640

Sigma-Aldrich

Tin, powder, <150 μm, 99.5% trace metals basis

266744

Sigma-Aldrich

Copper, foil, thickness 1.0 mm, 99.999% trace metals basis

267902

Sigma-Aldrich

Titanium, wire, diam. 0.81 mm, 99.7% trace metals basis