Skip to Content
Merck
  • Effect of irrigation with Tetraclean on bacterial leakage of obturated root canals.

Effect of irrigation with Tetraclean on bacterial leakage of obturated root canals.

The New York state dental journal (2014-06-18)
Zahed Mohammadi, Sousan Shalavi, Luciano Giardino, Flavio Palazzi
ABSTRACT

The purpose of the study presented here was to evaluate the effect of Tetraclean, Hypoclean, Chlor-XTRA, 2% chlorhexidine and 6% sodium hypochlorite/17% EDTA as a final irrigant on bacterial leakage of the root canal. One hundred and fifty-five extracted human maxillary central incisors were randomly divided into five experimental groups of 25 teeth each and two control groups of 15 teeth each. The root canals in each group were irrigated with 2 ml of the relevant irrigant between each filing. The root canals in group 5 were irrigated with 5 ml of 17% EDTA at the end of root canal preparation. The teeth in each group were obturated with gutta-percha and AH-26 sealer. Positive control teeth were obturated with a single gutta-percha cone without sealer, and negative controls were obturated in the same way as experimental groups. The coronal portion of each root was placed in contact with inoculum of Enterococcus faecalis in Brain Heart Infusion (BHI) culture media. Findings showed that the mean number of days for bacterial penetration in the Tetraclean group was greater than for other experimental groups. On the other hand, the Chlor-XTRA Vista group showed the fewest mean number of days for bacterial leakage.

MATERIALS
Product Number
Brand
Product Description

Supelco
Citric acid, Anhydrous, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Citric acid, ACS reagent, ≥99.5%
Sigma-Aldrich
Chlorhexidine dihydrochloride, ≥98%
Sigma-Aldrich
Ethylenediaminetetraacetic acid disodium salt solution, Molecular Biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected
Sigma-Aldrich
Citric acid, anhydrous, suitable for cell culture, suitable for plant cell culture
Sigma-Aldrich
Citric acid, 99%
Sigma-Aldrich
Ethylenediaminetetraacetic acid, anhydrous, crystalline, BioReagent, suitable for cell culture
Sigma-Aldrich
Ethylenediaminetetraacetic acid, purified grade, ≥98.5%, powder
Sigma-Aldrich
Ethylenediaminetetraacetic acid, BioUltra, anhydrous, ≥99% (titration)
Sigma-Aldrich
Ethylenediaminetetraacetic acid disodium salt solution, 2%, solution
Sigma-Aldrich
Ethylenediaminetetraacetic acid solution, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
Bismuth, granular, ≥99.99% trace metals basis
Sigma-Aldrich
Ethylenediaminetetraacetic acid, BioUltra, ≥99.0% (KT)
Sigma-Aldrich
Ethylenediaminetetraacetic acid, ≥98.0% (KT)
Titanium, IRMM®, certified reference material, 0.1 mm foil
Sigma-Aldrich
Ethylenediaminetetraacetic acid disodium salt solution, BioUltra, pH 8.0, ~0.5 M in H2O
Sigma-Aldrich
Citric acid, BioUltra, anhydrous, ≥99.5% (T)
Chlorhexidine dihydrochloride, European Pharmacopoeia (EP) Reference Standard
USP
Citric acid, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Ethylenediaminetetraacetic acid, 99.995% trace metals basis
Sigma-Aldrich
Citric acid, ≥99.5%, FCC, FG
Titanium, rod, 100mm, diameter 8.0mm, as drawn, 99.99+%
Silver, foil, 15mm disks, thickness 0.15mm, as rolled, 99.95+%
Titanium, tube, 500mm, outside diameter 10mm, inside diameter 8mm, wall thickness 1.0mm, annealed, 99.6+%
Titanium, wire reel, 10m, diameter 0.05mm, hard, 99.8%
Silver, foil, 15mm disks, thickness 0.1mm, annealed, 99.95+%
Titanium, tube, 500mm, outside diameter 1.6mm, inside diameter 1.2mm, wall thickness 0.2mm, hard, 99.6+%
Titanium, tube, 500mm, outside diameter 12.7mm, inside diameter 10.9mm, wall thickness 0.9mm, annealed, 99.6+%
Silver, foil, 15mm disks, thickness 0.006mm, 99.97%
Titanium, wire reel, 100m, diameter 0.5mm, as drawn, 99.6+%