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Rapid and mild purification method for nanoparticles from a dispersed solution using a monolithic silica disk.

Rapid and mild purification method for nanoparticles from a dispersed solution using a monolithic silica disk.

Journal of chromatography. A (2015-06-11)

Naoki Itoh, Tomofumi Santa, Masaru Kato

ABSTRACT

A rapid and mild purification method for nanoparticles using the commercially available monolithic silica disk, MonoSpin(®), was developed. The nanoparticles were purified from a dispersed solution by filtration with the aid of centrifugation at 2290×g for 2min. The purification conditions were rapid, mild, and simple compared with those of the conventional purification methods such as ultracentrifugation, dialysis, size exclusion chromatography, and ultrafiltration. The method was shown to be applicable for the purification of various nanoparticles, regardless of their size (from 21 to 100nm), composition material (silica, polyethylene glycol, and pegylated liposome), and encapsulated molecule (rhodamine 110 and doxorubicin). It was shown that this method is applicable to the purification of a wide range of nanoparticles in many different fields.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

L-Arginine, 99%, FCC, FG

Sigma-Aldrich

N,N,N′,N′-Tetramethylethylenediamine, ≥99.5%, purified by redistillation

Sigma-Aldrich

L-Arginine, reagent grade, ≥98%

Sigma-Aldrich

N,N,N′,N′-Tetramethylethylenediamine, BioReagent, suitable for electrophoresis, ≥99.0%

Sigma-Aldrich

L-Arginine, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%

Sigma-Aldrich

N,N,N′,N′-Tetramethylethylenediamine, BioReagent, Molecular Biology, ≥99% (GC)

Sigma-Aldrich

Ammonium persulfate, BioXtra, ≥98.0%

Sigma-Aldrich

Acetonitrile, anhydrous, 99.8%

SAFC

L-Arginine

Sigma-Aldrich

Ammonium persulfate, BioUltra, ≥98.0% (RT)

Sigma-Aldrich

Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer

Sigma-Aldrich

L-Arginine, BioUltra, ≥99.5% (NT)

Sigma-Aldrich

Ammonium persulfate, Molecular Biology, suitable for electrophoresis, ≥98%

Sigma-Aldrich

N,N,N′,N′-Tetramethylethylenediamine, ReagentPlus^®, 99%

Sigma-Aldrich

Acetonitrile, electronic grade, 99.999% trace metals basis

Sigma-Aldrich

Hydrogen chloride solution, 3 M in cyclopentyl methyl ether (CPME)

Sigma-Aldrich

Methanol, suitable for NMR (reference standard)

Sigma-Aldrich

Acryloyl chloride, 97.0%, contains <210 ppm MEHQ as stabilizer

Sigma-Aldrich

Acetic acid, ≥99.5%, FCC, FG

Sigma-Aldrich

Triethylamine, ≥99.5%

Sigma-Aldrich

Acryloyl chloride, ≥97%, contains ~400 ppm phenothiazine as stabilizer

Sigma-Aldrich

Acetic acid-¹²C₂, 99.9 atom % ¹²C

Sigma-Aldrich

Triethylamine, for amino acid analysis, ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, ampule, ≥99.5% (GC)

Sigma-Aldrich

Acetic acid, suitable for luminescence, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, puriss. p.a., ≥99.5% (GC)

Sigma-Aldrich

Hydrochloric acid solution, ~6 M in H₂O, for amino acid analysis

Sigma-Aldrich

Diethyl ether

Sigma-Aldrich

Acetic acid, natural, ≥99.5%, FG