The gelation process for this product does not require pH neutralization by the addition of acid or base. The procedure involves pipetting undiluted collagen into culture dishes, exposing them to ammonia vapor, and rinsing with sterile distilled water multiple times to remove ammonia. The final rinse is replaced with a sterile medium or balanced salt solution with serum, and the dishes are stored at 4°C until use.
08-115
Rat Collagen Type I
from rat tail, liquid, 2-5 mg/mL, suitable for cell culture, used for 3D gel formation
Synonym(s):
Rat tail collagen, Rat tail collagen type I
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€355.00
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100 MG
€355.00
About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
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Product Name
Collagen Type I, rat tail, 100 mg, solution, BioReagent, suitable for cell culture
biological source
rat
Quality Level
form
liquid
solution
manufacturer/tradename
Upstate®
concentration
100 mg
technique(s)
cell culture | mammalian: suitable (and for 3D proliferation assays.)
input
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type: mouse embryonic stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
Gene Information
rat ... COL1A1(29393)
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This Item | 11179179001 | 234138 | AB765P |
|---|---|---|---|
| biological source rat | biological source rat tail | biological source human skin | biological source rabbit |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) ELISA: suitable, immunohistochemistry: suitable, radioimmunoassay: suitable, western blot: suitable |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form liquid | form lyophilized (clear, colorless solution after reconstitution) | form lyophilized | form - |
| shipped in wet ice | shipped in - | shipped in ambient | shipped in dry ice |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - |
General description
In humans, type I collagen is the most abundant and broadly expressed collagen. This heterotrimer contains two alpha 1 (I) chains and one alpha 2 (I) chain.[1] Collagen type I α 1 (COL1A1), a major component of collagen type I, codes for pro-α 1 chains of type I collagen.[2] It has repeating Gly-Xaa-Yaa tri-peptides motifs. The collagen alpha-1(I) chain protein is a member of the collagen complex superfamily.[1] COL1A1 has a von Willebrand factor type C (vWFC) domain, a collagen triple-helix repeat, and a fibrillar collagen C-terminal domain (COLF).[2]
Application
Collagen Type I, rat tail has been used:
- for collagen 3D proliferation assays[1]
- for atelocollagen proliferation assays[1]
- in a comparative study to make microcapsules for templating on mesoporous cores of pure vaterite calcium carbonate (CaCO3) crystals to evaluate the structure-property relationship[2]
- in coverslip coating to grow the human cerebral microvascular endothelial cell line (hCMEC/D3) for creating a cell coculture and chronic subdural hematoma (CSDH) patient hematoma sample induced injury model[3]
Biochem/physiol Actions
Collagen type I α 1 (COL1A1) plays a key role in chemoresistance and may act as a therapeutic target. It is involved in carboplatin-resistant ovarian cancer (OV).[1] COL1A1 serves as an oncogene in the development of colorectal cancer (CRC).[2] Dominant inherited COL1A1 gene mutations result in connective tissue disorders such as osteogenesis imperfecta (OI).[1]
Quality
Routinely evaluated by testing for uniform gelation and for attachment of and spreading of PC-12 rat cells
Physical form
0.02N acetic acid, pH 3.67
Storage and Stability
1 year at 4°C
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Does this product require pH neutralization by addition of acid or base for gelation?
1 answer-
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Hi team: I hope this finds you well. May I know the concentration of collagen type I , rat tail 08-115? Much appreciated. Kind regards, Ovi
1 answer-
The concentration is provided in lot specific Certificates of Analysis, typically within the range of 3 to 5 mg/ml.
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Accidentally, I opened the collagen out of the flow chamber. Should I filter it or autoclave it to ensure the sterile conditions?
1 answer-
Collagen solutions may be sterilized by dialysis in a 0.25% acetic acid and 0.5% chloroform solution.
Avoid sterilizing the collagen solution by membrane filtration, as substantial protein loss may occur by this method. The coating of tissue culture plastic dishes may be performed by air-drying. Dried coated dishes may then be sterilized by exposure to UV light in a sterile culture hood or by rinsing with 70% ethanol.Helpful?
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Hi, what is the concentration of the collagen?
1 answer-
The concentration of this product will be listed on the lot-specific Certificate of Analysis. Please see the link below to access a Certificate:
https://www.emdmillipore.com/US/en/product/Collagen-Type-I-rat-tail,MM_NF-08-115#anchor_COAHelpful?
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Once diluted in 0.02N acetic acid, does the collagen solution require sterilisation for use in cell culture? If so, which method is the most effective: UV, autoclave or 0.22uM sterile filter (should this be PES or PVDF)?
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This product is supplied as a sterile solution in 0.02 N Acetic acid. The package size is 100 mg, however, the concentration is variable and reported on the lot-specific Certificate of Analysis. The typical range is 4 - 5 mg/mL. Dilutions can be made with sterile PBS. Further sterilization is not required. If necessary, coated slides and coverslips can be sterilized by UV.
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