AB8345

Anti-MMP-14 Antibody

serum, Chemicon^®

• Synonym(s): MT1-MMP
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NM_004995.2
• UniProt accession no.: P50281
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... MMP14(4323)

Description

Immunogen

Catalytic domain of MT1-MMP

Application

Anti-MMP-14 Antibody detects level of MMP-14 & has been published & validated for use in ELISA, FC, IP, WB & IC.

Immunocytochemistry: 5-10 μg / ml

Western blot: 5 μg / ml Expected band sizes: 60-63 kDA and 42 kDa pro-enzyme and degradation form respectively.

Immunopreciptation: 1-2 μg / mg total protein in 500 μl reaction volume.

FACS Analysis: 5 μg/ mL (1 million cells per mL)

EIA: 1:10,000

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Biochem/physiol Actions

The antibody recognizes native and recombinant proteins.

Physical form

Liquid in PBS, pH 7.6, containing 0.01% sodium azide as a preservative.

Preparation Note

Store antibody at -20°C for 12 months. Avoid repeat freeze thaw cycles.

Analysis Note

Control
Immunocytochemistry positive control: U251 glioma cells; negative control MCF7 breast carcinoma.

Western Blot positive control: HT1080 fibrosarcoma, negative control: MCF7 breast carcinoma

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable