MAC142

Anti-Cas9 Antibody, N-terminal Antibody, clone 6H4 Antibody, S. aureus

clone 6H4, from mouse

• Synonym(s): SaCas9, CRISPR-associated endonuclease Cas-9
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6H4, monoclonal
• species reactivity: Staphylococcus aureus, bacteria
• species reactivity (predicted by homology): all
• packaging: antibody small pack of 25 μg
• technique(s): immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2bκ
• UniProt accession no.: J7RUA5
• shipped in: ambient
• target post-translational modification: unmodified

Description

General description

CRISPR-associated endonuclease Cas9 (UniProt: J7RUA5; also known as SaCas9) is encoded by the Cas9 gene (Gene ID: in Staphylococcus aureus. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer.Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self-versus non-self, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity. Cas9 endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed 3′-5′ exonucleolytically.

~124 kDa observed; 123.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

Epitope: N-terminus

His-tagged recombiant Cas-9 from Staphylococcus aureus.

Application

Anti-Cas-9, N-terminal, clone 6H4, Cat. No. MAC142, is a mouse monoclonal antibody that detects Cas-9 in Staphylococcus aureus and its identity has been confimed by Isotyping Test and Western Blotting.

Research Category
Secondary & Control Antibodies

Western Blotting Analysis: 0.57 µg/mL from a representative lot detected CRISPR-associated endonuclease Cas9/Csn1 in whole cell lysates of HEK293 (WT), HEK293/Cas9 from S. aureus, or HeLa/Cas9 from S. pyogenes. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected CRISPR-associated endonuclease Cas9/Csn1 in transiently transfected U20S cells expressing SaCas9-T2A-GFP. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Immunoprecipitation Analysis: 1-2 µg from a representative lot detected CRISPR-associated endonuclease Cas9/Csn1 in SaCas9. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Biochem/physiol Actions

Clone 6H4 detects Cas-9 in Staphylococcus aureus.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable