Potassium phosphate monobasic

Name: Potassium phosphate monobasic
Brand: SIGMA

BioUltra, Molecular Biology, anhydrous, ≥99.5% (T)

Synonym(s):

Potassium dihydrogen phosphate, prim.-Potassium phosphate

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About This Item

Linear Formula:

KH₂PO₄

CAS Number:

7778-77-0

Molecular Weight:

136.09

NACRES:

NA.25

PubChem Substance ID:

57651733

UNSPSC Code:

12161700

EC Number:

231-913-4

MDL number:

MFCD00011401

Key Documents

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Product Name

Potassium phosphate monobasic, BioUltra, Molecular Biology, anhydrous, ≥99.5% (T)

InChI key

GNSKLFRGEWLPPA-UHFFFAOYSA-M

InChI

1S/K.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1

SMILES string

[K+].OP(O)([O-])=O

grade

Molecular Biology

product line

BioUltra

assay

≥99.5% (T)

form

powder or crystals

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.001% total nitrogen (N)

pH

4.0-4.5 (25 °C, 1 M in H₂O)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

mp

252.6 °C (lit.)

solubility

H₂O: 1 M at 20 °C, clear, colorless

density

2.338 g/mL at 25 °C (lit.)

anion traces

chloride (Cl^-): ≤5 mg/kg
sulfate (SO₄^2-): ≤30 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H₂O

UV absorption

λ: 260 nm A_max: 0.050
λ: 280 nm A_max: 0.045

Quality Level

200

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Related Categories

Biochemicals

Biological Buffers

High-Purity Biochemicals and Reagents

Phosphates

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Show Differences

1 of 4

This Item	60229	RDD037	P5655
Sigma-Aldrich 60218 Potassium phosphate monobasic	Sigma-Aldrich 60229 Potassium phosphate monobasic	Sigma-Aldrich RDD037 Potassium phosphate monobasic	Sigma-Aldrich P5655 Potassium phosphate monobasic
grade for molecular biology	grade anhydrous	grade -	grade -
assay ≥99.5% (T)	assay 98-100.5% dry basis	assay ≥99.0%	assay ≥99.0%
product line BioUltra	product line -	product line Redi-Dri^™	product line -
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 200
form powder or crystals	form crystalline	form -	form powder
solubility H₂O: 1 M at 20 °C, clear, colorless	solubility water: 208 g/L at 20 °C	solubility -	solubility water: 100 mg/mL, clear, colorless

Application

Potassium phosphate monobasic and dibasic, are used to create phosphate buffered solutions such as potassium phosphate buffered water and saline solutions used in biological applications.

Other Notes

Component of PBS - in the isolation and analysis of nuclear RNA.

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Isolation and analysis of nuclear RNA.

J R Nevins

Methods in enzymology, 152, 234-241 (1987-01-01)

Dystrophin proteolysis: a potential target for MMP-2 and its prevention by ischemic preconditioning.

Bruno Buchholz et al.

American journal of physiology. Heart and circulatory physiology, 307(1), H88-H96 (2014-05-06)

Dystrophin is responsible for the mechanical stabilization of the sarcolemma, and it has been shown that it is one of the most sensitive proteins to ischemic injury. However, the enzyme responsible for this proteolysis is still unknown. Isolated rabbit hearts

Concurrent speed endurance and resistance training improves performance, running economy, and muscle NHE1 in moderately trained runners.

Casper Skovgaard et al.

Journal of applied physiology (Bethesda, Md. : 1985), 117(10), 1097-1109 (2014-09-06)

The purpose of this study was to examine whether speed endurance training (SET, repeated 30-s sprints) and heavy resistance training (HRT, 80-90% of 1 repetition maximum) performed in succession are compatible and lead to performance improvements in moderately trained endurance

Novel recombinant multiepitope proteins for the diagnosis of asymptomatic leishmania infantum-infected dogs.

Angélica Rosa Faria et al.

PLoS neglected tropical diseases, 9(1), e3429-e3429 (2015-01-09)

Visceral leishmaniasis is the most severe form of leishmaniasis. Worldwide, approximately 20% of zoonotic human visceral leishmaniasis is caused by Leishmania infantum, also known as Leishmania chagasi in Latin America. Current diagnostic methods are not accurate enough to identify Leishmania-infected

Activation of diverse signalling pathways by oncogenic PIK3CA mutations.

Xinyan Wu et al.

Nature communications, 5, 4961-4961 (2014-09-24)

The PIK3CA gene is frequently mutated in human cancers. Here we carry out a SILAC-based quantitative phosphoproteomic analysis using isogenic knockin cell lines containing 'driver' oncogenic mutations of PIK3CA to dissect the signalling mechanisms responsible for oncogenic phenotypes induced by

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Questions

1–2 of 2 Questions

Anonymous

6 months ago

How can I determine the shelf life / expiration / retest date of this product?

1 answer
1. Technical Support
  
  6 months ago
  
  If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
  For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
  For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
  For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
  
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Anonymous

10 months ago

How is shipping temperature determined? And how is it related to the product storage temperature?

1 answer
1. Technical Support
  
  10 months ago
  
  Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
  
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