A5043

Adenosine Deaminase from bovine spleen

Name: Adenosine Deaminase from bovine spleen
Brand: SIGMA

Type X, buffered aqueous glycerol solution, ≥130 units/mg protein

Synonym(s):

ADA, adenosine aminohydrolase, Adenosine aminohydrolase

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About This Item

CAS Number:

9026-93-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.5.4.4 (BRENDA, IUBMB)

MDL number:

MFCD00081287

Key Documents

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Product Name

Adenosine Deaminase from bovine spleen, Type X, buffered aqueous glycerol solution, ≥130 units/mg protein

biological source

bovine spleen

type

Type X

form

buffered aqueous glycerol solution

specific activity

≥130 units/mg protein

mol wt

32.5-33 kDa

cation traces

NH₄⁺: ≤10 μg/mg protein

UniProt accession no.

P56658

foreign activity

alkaline phosphatase ≤0.01%
guanase ≤0.01%
nucleoside phosphorylase ≤0.1%

storage temp.

2-8°C

Quality Level

200

Gene Information

cow ... ADA(280712)

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This Item	A5168	P9041	L1006
Sigma-Aldrich A5043 Adenosine Deaminase from bovine spleen	Sigma-Aldrich A5168 Adenosine Deaminase from bovine spleen	Sigma-Aldrich P9041 Phosphodiesterase II from bovine spleen	Sigma-Aldrich L1006 L-Lactic Dehydrogenase from bovine heart
Gene Information cow ... ADA(280712)	Gene Information cow ... ADA(280712)	Gene Information cow ... PDE2A(281971)	Gene Information cow ... LDHA(281274), LDHB(281275)
biological source bovine spleen	biological source bovine spleen	biological source -	biological source bovine heart
specific activity ≥130 units/mg protein	specific activity 150-200 units/mg protein	specific activity ≥5.0 units/mg protein	specific activity ≥400 units/mg protein
form buffered aqueous glycerol solution	form ammonium sulfate suspension	form lyophilized powder	form buffered aqueous glycerol solution
UniProt accession no. P56658	UniProt accession no. P56658	UniProt accession no. P14099	UniProt accession no. P19858, Q5E9B1
mol wt 32.5-33 kDa	mol wt 32.5-33 kDa	mol wt 65 kDa	mol wt 140 kDa

Analysis Note

Protein determined by biuret.

Application

Adenosine Deaminase from bovine spleen has been used in the immobilization on biostrip for stability and catalytic studies.[1]

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays [2]. Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Biochem/physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

General description

Molecular weight: 32.5-33 kDa

pI: 4.85

Other Notes

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Physical form

Solution in 50% glycerol, 5 mM potassium phosphate, pH 6.0

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Adenosine reagent-free detection by co-immobilization of adenosine deaminase and phenol red on an optical biostrip

Bartzoka F, et al.

Biotechnology Journal, 10(1), 136-142 (2015)

Leucine deprivation decreases fat mass by stimulation of lipolysis in white adipose tissue and upregulation of uncoupling protein 1 (UCP1) in brown adipose tissue.

Ying Cheng et al.

Diabetes, 59(1), 17-25 (2009-10-17)

White adipose tissue (WAT) and brown adipose tissue (BAT) play distinct roles in adaptation to changes in nutrient availability, with WAT serving as an energy store and BAT regulating thermogenesis. We previously showed that mice maintained on a leucine-deficient diet

Neutropenia and myeloid dysplasia in a patient with delayed-onset adenosine deaminase deficiency.

Keiko Nomura et al.

Pediatric blood & cancer, 60(5), 885-886 (2013-01-22)

RNA-interacting proteins act as site-specific repressors of ADAR2-mediated RNA editing and fluctuate upon neuronal stimulation.

Aamira Tariq et al.

Nucleic acids research, 41(4), 2581-2593 (2013-01-01)

RNA editing by adenosine deaminases that act on RNA (ADARs) diversifies the transcriptome by changing adenosines to inosines. In mammals, editing levels vary in different tissues, during development, and also in pathogenic conditions. From a screen for repressors of editing

[Adenosine deaminase in severe combined immunodeficiency syndrome].

Mary Carmen Pérez-Aguilar et al.

Investigacion clinica, 53(3), 315-324 (2012-12-20)

Adenosine deaminase is an enzyme of the purine metabolism whose function is to convert adenosine to inosine and deoxyadenosine to deoxyinosine. The ecto-ADA1 binding to the cell surface through CD26 contributes to the regulation of cytokines and stimulates the proliferation

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