A6582

Antifoam A concentrate

active silicone polymer 100%

• Synonym(s): Antifoam agent, Antifoam solution, Foam control agent
• UNSPSC Code: 12161901
• NACRES: NA.85

Properties

• Quality Level: 200
• form: emulsion (aqueous)
• technique(s): cell culture | hybridoma: suitable; microbiological culture: suitable
• density: 0.97 g/mL at 25 °C
• application(s): microbiology
• storage temp.: room temp
• suitability: (Mammalian (suspension)); bacteria (fermentation)

Description

General description

Antifoam A is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production. Extremely effective for aqueous and non-aqueous systems. Effective at concentrations in the 1-50ppm range.

Application

Antifoam A concentrate has been used:

• in bioreactor culture for Staphylococcal enterotoxin C (SEC) production.[1]
• to prevent foam formation during the aerobic treatment of sludge. [2]
• to study the impact of surface tension on bioaerosol generation during bubbling. [3]

Features and Benefits

• Molecular biology grade and tested for use in bacterial fermentation.
• Extremely effective foam suppressor for aqueous and non-aqueous systems.
• Made of 100% active silicone polymer, free of emulsifiers.
• Typically, effective at 1-100 ppm.
• Product will be stable in the pH range of 5 to 9.
• Can be directly added to a fermentation medium but it is not recommended that it be pumped to a fermenter on an as-needed basis.

Preparation Note

Antifoam A can be pre-diluted with 3-10 parts of aqueous propylene glycol or non-aqueous vegetable oil with slow mixing.

Other Notes

Antifoam A is a 100% active silicon polymer containing no non-ionic emulsifiers .

Safety Information

• Pictograms: GHS09
• Signal Word: Warning
• Hazard Statements: H410
• Precautionary Statements: P273 - P391 - P501
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): >213.8 °F - closed cup
• Flash Point(C): > 101 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves