D0550

3,5-Dinitrosalicylic acid

Name: 3,5-Dinitrosalicylic acid
Brand: SIGMA

used in colorimetric determination of reducing sugars

Synonym(s):

3,5-Dinitro-2-hydroxybenzoic acid, DNS

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About This Item

Linear Formula:

(O₂N)₂C₆H₂-2-(OH)CO₂H

CAS Number:

609-99-4

Molecular Weight:

228.12

Beilstein:

2220661

EC Number:

210-204-3

MDL number:

MFCD00007104

UNSPSC Code:

12171500

PubChem Substance ID:

24893243

NACRES:

NA.47

Key Documents

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Assay

≥98% (HPLC and titration)

form

powder

mp

168-172 °C (lit.)

solubility

H₂O: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)

InChI key

LWFUFLREGJMOIZ-UHFFFAOYSA-N

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Show Differences

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This Item	128848	D124001	136883
Sigma-Aldrich D0550 3,5-Dinitrosalicylic acid	Sigma-Aldrich 128848 3,5-Dinitrosalicylic acid	Sigma-Aldrich D124001 3,5-Diiodosalicylic acid	Sigma-Aldrich 136883 4-Methyl-3,5-dinitrobenzoic acid
assay ≥98% (HPLC and titration)	assay 98%	assay 99%	assay 98%
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
solubility H₂O: 50 mg/mL	solubility -	solubility -	solubility methanol: soluble 250 mg/10 mL, clear, faintly yellow to yellow
storage temp. room temp	storage temp. -	storage temp. -	storage temp. -
form powder	form powder	form powder	form solid
mp 168-172 °C (lit.)	mp 168-172 °C (lit.)	mp 220-230 °C (dec.) (lit.)	mp 155-158 °C (lit.)

Application

3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study.[1] It has also been used for the quantitation of enzymatically released reducing sugars.[2]

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars[3][4] and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.[5][6]

Pictograms

GHS05,GHS07

Signal Word

Danger

Hazard Statements

H302,H318

Precautionary Statements

P264 - P270 - P280 - P301 + P312 - P305 + P351 + P338 - P501

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1

Storage Class Code

4.1A - Other explosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Comparison of the 3,5-dinitrosalicylicacid and Nelson-Somogyi methods of assaying for reducing sugars and determining cellulase activity.

Breuil, C. and Saddler, J.N.

Enzyme and Microbial Technology, 7, 327-332 (1985)

Studies on the ecology of actinomycetes in an agricultural soil amended with organic residues: II. Assessment of enzymatic activities of Actinomycetales isolates.

Mokni-Tlili S, et al.

World Journal of Microbiology & Biotechnology, 27, 2251-2259 (2011)

Use of dinitrosalicylic acid reagent for determination of reducing sugar.

Miller, G.L.

Analytical Chemistry, 31, 426-428 (1959)

Reducing value methods for maltodextrins. I. Chain-length dependence of alkaline 3,5-dinitrosalicylate and chain-length independence of alkaline copper.

J F Robyt et al.

Analytical biochemistry, 45(2), 510-516 (1972-02-01)

Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities.

Gusakov, A.V., et al.

International Journal of Antimicrobial Agents, e283658-e283658 (2011)

View All Related Papers

Protocols

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

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