One vial of Product D3687, DirectLoad™ PCR 100 bp Low Ladder, contains 375 µL.
D3687
DirectLoad™ PCR 100 bp Low Ladder
ready-to-use marker for DNA electrophoresis
Synonym(s):
100bp ladder for gel electrophoresis, 100bp maker, DirectLoad™ marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker
Sign In to View Organizational & Contract Pricing
Select a Size
Pricing and availability is not currently available.
About This Item
UNSPSC Code:
41105335
NACRES:
NA.31
Skip To
grade
Molecular Biology
Quality Level
form
liquid
usage
75 uses
solubility
water: soluble
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
1 of 4
This Item | P1473 | D3937 | P9577 |
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade - | grade - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| usage 75 uses | usage 75 uses | usage 100 uses | usage sufficient for 50 uses |
| suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) |
General description
Sigma′s DirectLoad™ PCR 100 bp Low Ladder contains 10 dsDNA fragments from 100 − 1,000 bases, evenly spaced in 100 bp repeats. Ladder should be loaded in a single lane on an agarose gel.
Application
Suitable for size determination of PCR generated DNA fragments with either agarose or polyacrylamide gels.
Features and Benefits
- Ready-to-load
- Easy-to-use
- Popular band sizes
Other Notes
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s DirectLoad™ PCR 100 bp Low Ladder is provided in a solution of 5% glycerol, 4.2 mM EDTA, 0.09% orange G, and 0.0125% xylene cyanol.
Legal Information
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
related product
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Protocols
JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Related Content
-
How many mL are in one vial of the 100 bp ready to use DNA ladder?
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service