The pH of DMEM F-12 can be adjusted to 6.9 - 7.1 using NaOH 1N or HCl 1N.
The pH of bicarbonate buffered solutions usually rises 0.1 - 0.2 units during filtration
It is recommended to add HCl or NaOH drop-wise while continuously stirring to avoid drastic pH swings. Use the smallest volume possible to avoid significantly altering the osmolarity of the medium. Maintain sterile conditions when adjusting pH for cell culture use.
D6421
DMEM/F-12
With HEPES, sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture
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Product Name
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: 0.055 g/L
L-glutamine: no
phenol red: yes
NaHCO3: yes
HEPES: 15 mM
glucose: 3.15 g/L
shipped in
ambient
storage temp.
2-8°C
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This Item | D8062 | D8437 | D8900 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility - |
| form liquid | form liquid | form liquid | form powder |
| components sodium pyruvate: 0.055 g/L | components glucose: 3.15 g/L | components glucose: 3.15 g/L | components sodium pyruvate: 0.055 g/L |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
General description
A measured mixture of chemically defined ingredients makes up a basal medium, which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/F-12 is a completely defined synthetic medium, which is protein-free.[1]
Application
DMEM:F12 is a 50:50 mixture of DMEM and Ham′s F12 media that has proven to be useful in a wide range of cell culture applications, especially when supplemented with fetal bovine serum (FBS). Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.
Preparation Note
Supplement with 0.365 g/L L-glutamine.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Nutrient Mixture F-12 Ham Formulation
Protocols
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May I confirm if it is acceptable to use 0.1 M HCl and 0.1 M NaOH to balance the pH of the medium (D6421)? Are there any specific recommendations or precautions for this adjustment process to maintain the integrity and performance of the medium?
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