F3651

Fluorescein 5(6)-isothiocyanate

≥90% purity (HPLC), powder

• Synonym(s): FITC, Fluorescein isothiocyanate
• Empirical Formula (Hill Notation): C₂₁H₁₁NO₅S
• CAS Number: 27072-45-3
• Molecular Weight: 389.38
• NACRES: NA.47
• PubChem Substance ID: 24894855
• UNSPSC Code: 12171500
• EC Number: 248-207-7
• MDL number: MFCD00005063
• Beilstein/REAXYS Number: 1407295

Properties

• Product Name: Fluorescein 5(6)-isothiocyanate, ≥90% (HPLC)
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• assay: ≥90% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: yellow to orange
• solubility: acetone: 1 mg/mL, clear, light yellow to yellow
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• Quality Level: 200

Description

Application

Fluorescein 5(6)-isothiocyanate has been used:

• for fluorescence labeling of bacteria[1]
• as a standard for calibration of fluorometer[2]
• for the labeling of exosomes[3]

For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.[4][5]

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3