The antibody concentration for Product No. H6908 is reported as a lot-specific value on the certificates of analysis. Please refer to the Certificate of Analysis for the lot received for the specific antibody concentration. Values for recent lots have ranged from 0.5 - 0.7 mg/mL.
H6908
Anti-HA antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti Ha, Anti Ha Antibody, Anti-Ha Antibody, HA Antibody - Anti-HA antibody produced in rabbit, Ha Antibody, Ha Antibody Sigma, Anti-HA
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Product Name
Anti-HA antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
immunoprecipitation (IP): 2.5-4 μg/test using HA-tagged fusion protein from cell lysates
indirect immunofluorescence: 10-20 μg/mL using HA-tagged fusion protein transfected cells
western blot: 0.5-0.8 μg/mL using HA-tagged fusion protein transfected cell extracts
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
1 of 4
This Item | H3663 | H9658 | H9037 |
|---|---|---|---|
| antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form ascites fluid | antibody form purified from hybridoma cell culture |
| biological source rabbit | biological source mouse | biological source mouse | biological source mouse |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate TRITC conjugate |
| clone polyclonal | clone HA-7, monoclonal | clone HA-7, monoclonal | clone HA-7, monoclonal |
| form buffered aqueous solution | form - | form - | form buffered aqueous solution |
Application
Anti-HA antibody is suitable for use in immunoprecipitation and western blot.
It is also suitable for indirect immunofluorescence (10-20μg/mL using HA-tagged fusion protein transfected cells), immunoprecipitation (2.5-4μg/test using HA-tagged fusion protein from cell lysates), and western blot (0.5-0.8μg/mL using HA-tagged fusion protein transfected cell extracts).
It is also suitable for indirect immunofluorescence (10-20μg/mL using HA-tagged fusion protein transfected cells), immunoprecipitation (2.5-4μg/test using HA-tagged fusion protein from cell lysates), and western blot (0.5-0.8μg/mL using HA-tagged fusion protein transfected cell extracts).
Biochem/physiol Actions
Anti-HA antibody is specific for N- or C-terminal HA-tagged fusion proteins.
Hemagglutinin (HA) is an influenza-virus glycoprotein that regulates membrane fusion and receptor-binding functions during viral entry and infection. The virus gain entry into the host cell via endocytosis and successive membrane fusion mediated by the HA antigen. HA plays a crucial role in viral pathogenesis and host response to viral infection.
The HA gene is often used as an affinity tag for target proteins in recombinant expression vector systems. The HA-tagged proteins produced from such systems yield stable fusion products that do not interfere with the function and distribution of target proteins. Anti-HA antibody may detect cross-reacting bands in certain mammalian cells. The immunizing HA peptide (Product No.I2149) specifically inhibits the staining of the HA-tagged protein band. The nucleic acid sequence encoding the HA peptide has been incorporated into various expression plasmids adjacent to the cloning site thus enabling the cloning and expression of HA-tagged fusion protein. Such fusion proteins may be expressed in cells of various organisms: bacteria, yeast, insects and mammals. In the fusion protein, the HA sequence may serve as a recognition target for specific antibodies thus enabling detection, subcellular localization, characterization, quantification, functional analysis and affinity purification of the HA-tagged protein and associated bound proteins. Insertion of the HA epitope in different regions of a cellular protein followed by examination of the immunoreactivity of the epitope in intact and in permeabilized cells is useful for studying the cellular expression levels, topology and functional activity of the tagged protein. Antibody mediated detection of the HA tag obviates the need for time consuming generation of antibody specific for newly identified, low abundance, unstable, difficult to purify, cross-reactive or poorly immunogenic proteins.
The HA gene is often used as an affinity tag for target proteins in recombinant expression vector systems. The HA-tagged proteins produced from such systems yield stable fusion products that do not interfere with the function and distribution of target proteins. Anti-HA antibody may detect cross-reacting bands in certain mammalian cells. The immunizing HA peptide (Product No.I2149) specifically inhibits the staining of the HA-tagged protein band. The nucleic acid sequence encoding the HA peptide has been incorporated into various expression plasmids adjacent to the cloning site thus enabling the cloning and expression of HA-tagged fusion protein. Such fusion proteins may be expressed in cells of various organisms: bacteria, yeast, insects and mammals. In the fusion protein, the HA sequence may serve as a recognition target for specific antibodies thus enabling detection, subcellular localization, characterization, quantification, functional analysis and affinity purification of the HA-tagged protein and associated bound proteins. Insertion of the HA epitope in different regions of a cellular protein followed by examination of the immunoreactivity of the epitope in intact and in permeabilized cells is useful for studying the cellular expression levels, topology and functional activity of the tagged protein. Antibody mediated detection of the HA tag obviates the need for time consuming generation of antibody specific for newly identified, low abundance, unstable, difficult to purify, cross-reactive or poorly immunogenic proteins.
The antibody reacts specifically with HA-tagged fusion proteins by immunoblotting and immunoprecipitation.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
The hemagglutinin (HA) gene is mapped to segment 4 of Influenza B viral genome. HA is a dominant antigen present on the influenza viral surface. HA is a homotrimer, in which each monomer is produced as a single polypeptide. This monomer is cleaved into two subunits HA1 and HA2 by the host protease.
Influenza hemagglutinin protein is a nonapeptide derived from the major spike membrane glycoprotein of the human influenza virus. This strain specific glycoprotein is a homotrimer of 84 kDa monomers. Each monomers contain two disulfide-linked subunits: HA1 and HA2.
Anti-HA is produced in rabbit using a synthetic peptide corresponding to amino acid residues of the human Influenza hemagglutinin (HA), conjugated to KLH. The antibody is affinity-purified on the immobilized immunizing peptide.
Anti-HA antibody is specific for N- or C-terminal HA-tagged fusion proteins. The product may detect cross-reacting bands in certain mammalian cells. The immunizing HA peptide (Product No.I2149) specifically inhibits the staining of the HA-tagged protein band.
Influenza hemagglutinin protein is a nonapeptide derived from the major spike membrane glycoprotein of the human influenza virus. This strain specific glycoprotein is a homotrimer of 84 kDa monomers. Each monomers contain two disulfide-linked subunits: HA1 and HA2.
Anti-HA is produced in rabbit using a synthetic peptide corresponding to amino acid residues of the human Influenza hemagglutinin (HA), conjugated to KLH. The antibody is affinity-purified on the immobilized immunizing peptide.
Anti-HA antibody is specific for N- or C-terminal HA-tagged fusion proteins. The product may detect cross-reacting bands in certain mammalian cells. The immunizing HA peptide (Product No.I2149) specifically inhibits the staining of the HA-tagged protein band.
Immunogen
Synthetic peptide corresponding to amino acid residues of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Preparation Note
For continuous use, store at 2-8 °C for up to one month.
For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.
For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Instructions
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What is the concentration of Product No. H6908, anti HA?
1 answer-
Helpful?
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Is the immunizing peptide for Product H6908, Anti-HA antibody, available?
1 answer-
The immunizing peptide is Product No. I2149. It is useful for displacement of HA-tagged fusion proteins from HA antibody in immunoassays. The successful inhibition of antibody binding by HA peptide demonstrates the binding of the antibody is specific.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What strain of influenza hemagglutinin does Product H6908, Anti-HA antibody, recognize?
1 answer-
The sequence of the immunizing peptide is YPYDVPDYA. This sequence corresponds to amino acids 99-107 of human influenza virus X47 (classified as an H3N2 strain) referred to in Niman, H.L., et al., Proc. Natl. Acad. Sci. USA, 80, 4949-4953, 1983. However, according to the NCBI databases, the same epitope (YPYDVPDYA) corresponds to amino acids 98-106 in the vast majority of prevalent influenza viruses hence our claim in the data sheet of the antibodies. NCBI is National Center for Biotechnology Information.
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