For products requiring long-term storage in a freezer, labels indicate -20 °C. Typically, freezer facilities used for storing these products are thermostatically controlled to maintain temperatures within -10 to -25 °C. This range aligns with the recommended freezer storage conditions indicated in the United States Pharmacopeia.
P0609
Pepsin−Agarose from porcine gastric mucosa
lyophilized powder, ≥30 units/mg dry solid
Synonym(s):
Pepsin A
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Product Name
Pepsin−Agarose from porcine gastric mucosa, lyophilized powder, ≥30 units/mg dry solid
biological source
Porcine gastric mucosa
form
lyophilized powder
specific activity
≥30 units/mg dry solid
mol wt
35 kDa
matrix
4% cross-linked beaded agarose
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
pig ... LOC396892(396892)
Related Categories
1 of 4
This Item | 77161 | 77160 | 77151 |
|---|---|---|---|
| specific activity ≥30 units/mg dry solid | specific activity - | specific activity ≥500 U/mg | specific activity 1200-2400 U/mg |
| Gene Information pig ... LOC396892(396892) | Gene Information pig ... LOC396892(396892) | Gene Information pig ... LOC396892(396892) | Gene Information pig ... LOC396892(396892) |
| biological source Porcine gastric mucosa | biological source - | biological source - | biological source - |
| form lyophilized powder | form powder | form powder | form powder |
| mol wt 35 kDa | mol wt 35 kDa | mol wt 35 kDa | mol wt 35 kDa |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
Application
Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin. [1]
Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also been used for the preparation of F(ab′)2 fragments from rabbit IgG.
Used to produce F(ab′)2 fragments of antibodies.1
Biochem/physiol Actions
Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. pH optimum 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH > 6.
General description
Pepsin is an enzyme found in gastric secretion. [2]
Other Notes
One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)
Physical form
Lyophilized powder stabilized with lactose
Preparation Note
One gram solid swells to 5-10ml packed gel.
related product
Product No.
Description
Pricing
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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M Feldman et al.
Gastroenterology, 110(4), 1043-1052 (1996-04-01)
Recent studies suggesting that gastric secretion does not decrease with aging included few elderly individuals and measured only acid secretion. The aims of this study were to measure gastric acid and pepsin output in 206 health Americans (age range, 18-98
Ludmila Tucková et al.
Journal of leukocyte biology, 71(4), 625-631 (2002-04-03)
Celiac disease, induced by dietary gluten, is characterized by mucosal atrophy and local inflammation associated with cell infiltration and activation. Unlike other food proteins, gluten and its proteolytic fragments, besides inducing a specific immune response, were shown to activate components
Thomas A Kraus et al.
The Journal of clinical investigation, 115(8), 2234-2243 (2005-07-26)
To explore the requirement for M cells and the Peyer's patch (PP) in induction of oral tolerance and address the potential in vivo role of intestinal epithelial cells as nonprofessional APCs, we have attempted to induce tolerance in mice with
Katrine Lindholm Bøgh et al.
International archives of allergy and immunology, 161(1), 21-36 (2012-12-22)
It is generally believed that protein hydrolysis in the gastrointestinal tract decreases the allergenicity of food allergens. However, it remains unknown if specific properties of digestion products determine whether a sensitisation or tolerogenic immune response will develop. We sought to
K L Bøgh et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 39(10), 1611-1621 (2009-08-20)
Food allergies are a public health issue of growing concern, with peanuts in particular being associated with severe reactions. The peanut allergen, Ara h 1, belongs to the cupin plant food allergen family, which, unlike other structural families, appears to
Articles
Antibody fragmentation with our pepsin digestion protocol for IgG antibody fragmentation and preparation of F(ab’).
Protocols
This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.
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Instructions
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It is impossible to store a reagent at -20. freezer's have an operating window of a few degrees either side of the temperature it is set at therefore it is not an obtainable storage requirement. please can you advise a range that is suitable for storage.
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