Elemental analysis of this material has not been performed. The sulfate content is not determined.
P8169
Phytagel™
BioReagent, suitable for plant cell culture, powder
Phytagel™
Synonym(s):
Gellan Gum, Agar substitute gelling agent
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About This Item
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product line
BioReagent
form
powder
technique(s)
cell culture | plant: suitable
application(s)
agriculture
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This Item | G1910 | A9799 | A4550 |
|---|---|---|---|
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
| form powder | form - | form powder | form powder |
| technique(s) cell culture | plant: suitable | technique(s) - | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| product line BioReagent | product line - | product line - | product line - |
General description
Typical working concentration: 1.5-2.5 g/L in plant tissue culture media; up to 10 g/L in microbiological media. Phytagel™ requires the presence of cations (especially divalent) for gelling to occur. Concentrations of calcium and magnesium contained in most plant tissue culture media are typically sufficient for gelation. Low-salt media formulations, especially those used in microbiological applications, may require supplementation with additional calcium or magnesium salts (e.g., CaCl2 or MgSO4) or higher concentrations of Phytagel.
Application
Biochem/physiol Actions
Phytagel is intended for use in place of agar and other gelling agent for plant tissue culture. Phytagel has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets.
Preparation Note
To prevent clumping, add slowly to rapidly stirring medium prior to heating.
Other Notes
An agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination.
Legal Information
Phytagel is a trademark of Sigma-Aldrich Co. LLC
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Christine Le Roux et al.
Applied and environmental microbiology, 75(24), 7752-7759 (2009-10-27)
In the context of an increasing utilization of the interspecific hybrid Acacia mangium x A. auriculiformis as a plantation tree in the tropical humid zone, its symbiotic characterization was carried out in comparison with that of its two parental species.
Yasuka L Yamaguchi et al.
Frontiers in plant science, 8, 1195-1195 (2017-07-28)
Developmental plasticity is one of the most striking features of plant morphogenesis, as plants are able to vary their shapes in response to environmental cues. Biotic or abiotic stimuli often promote organogenesis events in plants not observed under normal growth
CEP genes regulate root and shoot development in response to environmental cues and are specific to seed plants
Delay C, et al.
Journal of Experimental Botany, 64(17), 5383-5394 (2013)
Micropropagation of Ilex dumosa (Aquifoliaceae) from nodal segments in a tissue culture system
Luna C, et al.
Biocell, 27(2), 205-212 (2003)
Abhishek Bhaskaran et al.
Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology, 19(5), 874-880 (2016-05-22)
Longer procedural time is associated with complications in radiofrequency atrial fibrillation ablation. We sought to reduce ablation time and thereby potentially reduce complications. The aim was to compare the dimensions and complications of 40 W/30 s setting to that of
Protocols
Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.
Related Content
Instructions
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What is the sulfate content, if any, of Phytagel?
1 answer-
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Buongiorno. Vorrei sapere se il Phytagel è Gellano ad alto o a basso grado di acilazione
1 answer-
This product is characterized as having a low acyl content.
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What is the molecular weight of one repeating unit?
1 answer-
The molecular weight is not determined. However, the constituent sugars of Phytagel are glucose, glucuronic acid and rhamnose in the molar ratio of 2:1:1.
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What is the gelling strength of Phytagel P8169
1 answer-
The Gel Strength for Phytagel (P8169) is 700 g/cm2 (tested at 0.5%).
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