14-751-M

Abl (Q252H) Protein, active, 10 µg

Name: Abl (Q252H) Protein, active, 10 µg
Brand: MM

Active, N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the Q252H mutation. For use in Kinase Assays.

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Código UNSPSC:

12352202

eCl@ss:

32160405

NACRES:

NA.32

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origen biológico

human

Nivel de calidad

100
300

recombinante

expressed in baculovirus infected Sf21 cells

actividad específica

1089 U/mg

mol peso

M_w 121 kDa

fabricante / nombre comercial

Upstate^®

concentración

51% (Coomassie staining, SDS-PAGE)

técnicas

activity assay: suitable (kinase)

Nº de acceso NCBI

NM_005157.3
NM_007313.2

Nº de acceso UniProt

P00519

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Este artículo	14-750-M	14-757	14-529-M
Sigma-Aldrich 14-751-M Abl (Q252H) Protein, active, 10 µg	Sigma-Aldrich 14-750-M Abl (H396P) Protein, active, 10 µg	Sigma-Aldrich 14-757 Abl (M351T) Protein, active, 10 µg	Sigma-Aldrich 14-529-M Abl Protein, active, 10 µg
recombinant expressed in baculovirus infected Sf21 cells	recombinant expressed in baculovirus infected Sf21 cells	recombinant expressed in baculovirus infected Sf21 cells	recombinant expressed in baculovirus infected Sf21 cells
biological source human	biological source human	biological source human	biological source human
technique(s) activity assay: suitable (kinase)	technique(s) activity assay: suitable (kinase)	technique(s) activity assay: suitable (kinase)	technique(s) activity assay: suitable (kinase)
concentration 51% (Coomassie staining, SDS-PAGE)	concentration -	concentration -	concentration -
mol wt M_w 121 kDa	mol wt M_w 121 kDa	mol wt M_w 121 kDa	mol wt M_w 121 kDa
UniProt accession no. P00519	UniProt accession no. P00519, U07563	UniProt accession no. P00519, U07563	UniProt accession no. P00519

Descripción general

N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the Q252H mutation. The Abl tyrosine kinase inhibitor STI571 is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the Q252H mutation, which confers resistance to drug binding (Shah et al., (2002), Cancer Cell 2, 117-125).

Product Source: Expressed by baculovirus in Sf21 insect cells.

Aplicación

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

Acciones bioquímicas o fisiológicas

Protein Target: Abl

Target Sub-Family: TK

Forma física

10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 150 mM NaCl, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1 mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution.

Ni²⁺/NTA-agarose

Nota de preparación

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Nota de análisis

Routinely evaluated by phosphorylation of 50 μM Abltide. Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active Abl (Q252H).

Otras notas

Specific Activity: one unit of Abl (Q252H) activity is defined as 1 nmol phosphate incorporated into 50 μM Abltide (EAIYAAPFAKKK) per minute at 30°C with a final ATP concentration of 100 μM.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogramas

GHS07

Palabra de señalización

Warning

Frases de peligro

H317

Consejos de prudencia

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Clasificaciones de peligro

Skin Sens. 1

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)