Anti-trimethyl Histone H3 (Lys27) Antibody

from rabbit, purified by affinity chromatography

H3K27me3, Histone H3 (tri methyl K27), H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3K27me3

origen biológico


Nivel de calidad


antibody product type

primary antibodies



purificado por

affinity chromatography

species reactivity

mouse, human


dot blot: suitable
immunocytochemistry: suitable
inhibition assay: suitable (peptide)
western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

wet ice

Gene Information

human ... HIST1H3F(8968)

Descripción general

Histone H3 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 lysine 27 methylation (H3K27) is associated with heterochromatin formation and transcriptional repression.


Broad species cross-reactivity expected.
This antibody recognizes Histone H3 methylated at Lys27.


Branch peptide corresponding to human Histone H3 methylated at Lys27.
Epitope: Lys27


Research Sub Category
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in NIH/3T3 cells.
Peptide Inhibition Assay: This antibody peptide blocked on HeLa cell extracts.
Anti-trimethyl Histone H3 (Lys27) Antibody is a rabbit polyclonal antibody for detection of Histone H3 trimethylated on lysine 27( H3K27me3). This purified polyclonal is specificity verified by dot blot (DB), published in peer reviewed journals and validated in WB, ICC, PIA.


Evaluated by Western Blot in HeLa acid extract and recombinant Histone H3.

Western Blot Analysis: 0.1 µg/ml of this antibody detected histone H3 in 10 µg of HeLa acid extract .

Descripción de destino

~ 17 kDa

Forma física

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Affinity Purfied

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

HeLa acid extract and recombinant Histone H3

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Alemania


Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis
Chromatin signature of widespread monoallelic expression.
Nag, A; Savova, V; Fung, HL; Miron, A; Yuan, GC; Zhang, K; Gimelbrant, AA
eLife null
Chromatin Signature Identifies Monoallelic Gene Expression Across Mammalian Cell Types.
Nag, A; Vigneau, S; Savova, V; Zwemer, LM; Gimelbrant, AA
G3 (Bethesda, Md.) null
Emily Maclary et al.
Nature communications, 5, 4209-4209 (2014-07-01)
Imprinted X-inactivation is a paradigm of mammalian transgenerational epigenetic regulation resulting in silencing of genes on the paternally inherited X-chromosome. The preprogrammed fate of the X-chromosomes is thought to be controlled in cis by the parent-of-origin-specific expression of two opposing...
Development of multiple cell-based assays for the detection of histone H3 Lys27 trimethylation (H3K27me3).
Qian, J; Lu, L; Wu, J; Ma, H
Assay and Drug Development Technologies null
Hao-Kang Li et al.
Scientific reports, 6, 28637-28637 (2016-06-28)
Studies indicate that the presence of cancer stem cells (CSCs) is responsible for poor prognosis of hepatocellular carcinoma (HCC) patients. In this study, the functional role of DDX3 in regulation of hepatic CSCs was investigated. Our results demonstrated that reduced...

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