The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals, which include notch-1. notch-2, notch-3, and notch-4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of notch-1. Mature notch proteins are heterodimeric receptors derived from the cleavage of notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).
This antibody recognizes Notch 1.
GST-tagged recombinant protein corresponding to the extracellular domain of rat Notch 1.
Epitope: Extracellular domain
Anti-Notch 1 Antibody, extracellular detects level of Notch 1 & has been published & validated for use in WB, IH(P).
Research Sub Category
Growth Factors & Receptors
Immunohistochemistry Analysis: 1:400 dilution of this antibody has been shown to detect Notch 1 in gastrointestinal and colorectal carcinoma tissue.
Evaluated by Western Blot in human lymph node lysate.
Western Blot Analysis: 1:500 dilution of this antibody detected Notch 1 on 10 µg of human lymph node lysate.
Descripción de destino
~260 kDa observed. Notch 1, extracellular is calculated at ~183 kDa
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Almacenamiento y estabilidad
Stable for 1 year at 2-8°C from date of receipt.
Nota de análisis
Human lymph node lysate
Cláusula de descargo de responsabilidad
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