APT110
Apo-Direct TUNEL Assay Kit
The APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.
Sinónimos:
Apoptosis detection kit, TUNEL apoptosis assay, TUNEL assay kit
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
50 assays | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 851,00 € |
Acerca de este artículo
UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000
851,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarleQuality Segment
species reactivity
vertebrates
manufacturer/tradename
ApoDIRECT, Chemicon®
technique(s)
flow cytometry: suitable
detection method
fluorometric
shipped in
wet ice
General description
The CHEMICON APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains all the reagents required for measuring apoptosis in cells including positive and negative control cells; washing, reaction, and rinsing buffers; and propidium iodide/RNase A solution for counter staining the total DNA.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
The APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.
Preparation Note
Precautions and Warnings:
1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.
2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid areas of contact immediately. See Material Safety Data Sheets.
3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.
1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.
2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid areas of contact immediately. See Material Safety Data Sheets.
3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.
Other Notes
The following components are included with the APO-DIRECT Kit. Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 50 cell suspensions including control cells of approximately 1 x 106 cells per suspension for flow cytometry analysis. The control cells have been fixed as described (under the heading cell fixation procedure) and are in 70% (v/v) ethanol.
Positive Control Cells,Brown cap
Negative Control Cells, Natural cap
Wash Buffer, Blue cap
Reaction Buffer, Green cap
TdT Enzyme, Yellow cap
F-dUTP, Orange cap
Rinsing Buffer, Red cap
PI/RNase Staining Buffer, Amber bottle
Positive Control Cells,Brown cap
Negative Control Cells, Natural cap
Wash Buffer, Blue cap
Reaction Buffer, Green cap
TdT Enzyme, Yellow cap
F-dUTP, Orange cap
Rinsing Buffer, Red cap
PI/RNase Staining Buffer, Amber bottle
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Este artículo | |||
|---|---|---|---|
| description The APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry. | description sufficient for 30 tests, pkg of 3 × 550 μL | description from calf thymus recombinant in E. coli | description sufficient for 100 fluorometric tests |
| technique(s) flow cytometry: suitable | technique(s) - | technique(s) - | technique(s) - |
| species reactivity vertebrates | species reactivity - | species reactivity - | species reactivity - |
| manufacturer/tradename Chemicon®, ApoDIRECT | manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename - |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| detection method fluorometric | detection method - | detection method - | detection method fluorometric |
| Quality Level 100 | Quality Level - | Quality Level - | Quality Level - |
Palabra de advertencia
Danger
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Carc. 1B - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1
Clase de almacenamiento
3 - Flammable liquids
Punto de inflamación (°F)
69.8 °F
Punto de inflamación (°C)
21 °C
¿Qué está pasando?
WGK 3
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