CC12

Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6)

liquid, clone DCS-6, Calbiochem^®

• Sinónimos: Anti-Bcl-1, Anti-PRAD-1
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: DCS-6, monoclonal
• Formulario: liquid
• contiene: ≤0.1% sodium azide as preservative
• reactividad de especies: human, mouse, rat
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: do not freeze
• dilution: (Flow Cytometry (5 µg/mL); Frozen Sections ; Immunoblotting (1 µg/mL); Immunofluorescence (5 µg/mL); Immunoprecipitation ; Neutralization Studies ; Paraffin Sections (5 µg/mL, heat pre-treatment required))
• isotipo: IgG2a
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CCND1(595) ; mouse ... Ccnd1(12443) ; rat ... Ccnd1(58919)

Descripción

Descripción general

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS/1 mouse myeloma cells. Recognizes the cyclin D1 protein.

Recognizes cyclin D1 in MCF-7 and A431 cells. Does not react with cyclins D2 and D3.

This Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of Cyclin D1 (Ab-3).

Inmunógeno

Human

recombinant human cyclin D1

Aplicación

Flow Cytometry (5 µg/ml; Cat. No. CC12, CC12F)

Frozen Sections (see application references)

Immunoblotting (1 µg/ml)

Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)

Immunoprecipitation (see application references)

Neutralization Studies (see application references)

Paraffin Sections (5 µg/ml, heat pre-treatment required)

Envase

Please refer to vial label for lot-specific concentration.

Forma física

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Nota de análisis

Negative Control
trp E (Ab-1)

Positive Control
MCF7, or A431 cells or breast carcinoma tissue

Otras notas

Does not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems.

Inaba, T., et al. 1992. Genomics13, 565.
Jiang, W., et al. 1992. Cancer Res.2, 2980.
Pines, J. 1992. Cell Growth Differ.2, 305.
Xiong, Y., et al. 1992. Genomics13, 575.
Xiong, Y., et al. 1992. Cell71, 504.
Motokura, T., et al. 1991. Nature350, 512.
Solomon, M.J., et al. 1990. Cell63, 1013.
Draetta, G., and Beach, D. 1988. Cell54, 17.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Toxicity: Standard Handling (A)

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable