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E5014

Benzonase ® Salt Tolerant Endonuclease

≥200 U/uL, for nucleic acid removal at high salt concentration

Sinónimos:

Endonuclease, nuclease

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Talla/SKUDisponibilidadPrecio
10 kU

Fecha estimada de envío04 de noviembre de 2026desdeMerck Chemicals and LS SA

294,00 €
40 kU

Fecha estimada de envío23 de octubre de 2026desdeMerck Chemicals and LS SA

1060,00 €

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UNSPSC Code:
12352204
NACRES:
NA.21
Form:
buffered aqueous glycerol solution
Biological source:
Escherichia coli (recombinant, expressed in E.coli)
Mol wt:
calculated mol wt 27 kDa

294,00 €

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biological source

Escherichia coli (recombinant, expressed in E.coli)

Quality Segment

form

buffered aqueous glycerol solution

mol wt

calculated mol wt 27 kDa

concentration

≥200 units/μL

input

general use sample(s)

storage temp.

-10 to -25°C

General description

The best-in-class solution for nucleic acid removal at high salt concentration. The Benzonase® Salt Tolerant endonuclease is an enzyme, different in amino acids sequence from other Benzonase® endonuclease references. It was designed with state-of-the-art protein engineering capabilities, to ensure the highest activity at high salt concentrations. The protein is a monomer with a molecular weight of about 27 kDa . The protein has an isoelectric point (pI) at pH 9.68. It is functional between pH 6 and 10, and from 0 °C to above 37 °C. Mg2+ (1–2 mM) is required for enzyme activity.

Application

Benzonase® Salt Tolerant endonuclease degrades both DNA and RNA, whether single stranded, double-stranded, linear, circular or supercoiled. No base preference is observed. As with all endonucleases, Benzonase® Salt Tolerant endonuclease hydrolyzes internal phosphodiester bonds present between the nucleotides. Upon complete digestion, all free nucleic acids present in solution are reduced to 5’-monophosphate terminated oligonucleotides, which are three to five bases in length.

Features and Benefits

  • Active at higher salt concentration, up to1M NaCl
  • Non-animal origin, recombinant from E.Coli, High Purity
  • No post-translational modifications
  • High batch to batch reproducibility
  • Free of detectable protease activity
  • Does not contain any antimicrobial preservatives or protein stabilizers except glycerol
  • Compatible with Deviron® detergents for cell lysis

Preparation Note

-10°C to -25°C

Legal Information

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
DEVIRON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Este artículo
SRE0116E1014SRE0115
vibrant-m

E5014

Benzonase ® Salt Tolerant Endonuclease

Salt Active Nuclease (SAN) recombinant, expressed in Pichia pastoris, Triton-free

Sigma-Aldrich

SRE0116

Salt Active Nuclease (SAN)

Nucleasas Benzonase® ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

Millipore

E1014

Nucleasas Benzonase®

Salt Active Nuclease (SAN) Heat-labile, recombinant, expressed in Pichia pastoris, Triton-free

Sigma-Aldrich

SRE0115

Salt Active Nuclease (SAN)

description

≥200 U/uL, for nucleic acid removal at high salt concentration

description

recombinant, expressed in Pichia pastoris, Triton-free

description

≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

description

Heat-labile, recombinant, expressed in Pichia pastoris, Triton-free

biological source

Escherichia coli (recombinant, expressed in E.coli)

biological source

-

biological source

Serratia marcescens

biological source

-

concentration

≥200 units/μL

concentration

-

concentration

≥250 units/μL

concentration

-

form

buffered aqueous glycerol solution

form

-

form

buffered aqueous glycerol solution

form

-

mol wt

calculated mol wt 27 kDa

mol wt

-

mol wt

30 kDa

mol wt

-

storage temp.

-10 to -25°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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