IM35

Sigma-Aldrich

Anti-MMP-1 (Ab-1) Mouse mAb (41-1E5)

liquid, clone 41-1E5, Calbiochem®

storage conditions

-20C

Nivel de calidad

100

origen biológico

mouse

antibody product type

primary antibodies

clon

41-1E5, monoclonal

formulario

liquid

contiene

≤0.1% sodium azide as preservative

species reactivity

human

should not react with

bovine

manufacturer/tradename

Calbiochem®

condiciones de almacenamiento

OK to freeze
avoid repeated freeze/thaw cycles

isotipo

IgG2a

enviado en

wet ice

temp. de almacenamiento

−20°C

Descripción general

Recognizes the ~55 kDa latent and the ~43 kDa active forms of MMP-1.
Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis.MMP-1 (interstitial collagenase, tissue collagenase, fibroblast collagenase) is secreted as a 57/52 kDa zymogen which is proteolytically processed to the 46/42 kDa active forms. This enzyme displays substrate specificity toward type I, II, III, VII, VIII and X collagens and gelatin. MMP-1 is thought to play an important role in pathophysiological degradation processes associated with conditions such as rheumatoid arthritis, osteoarthritis, and cancer cell invasion.
Purified mouse monoclonal antibody. Recognizes the ~55 kDa latent and the ~43 kDa active forms of MMP-1.

Inmunógeno

Human
a synthetic peptide (VQGQNVLHGYPKDIYSSFG) corresponding to amino acids 332-350 of human MMP-1

Envase

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Aplicación


Frozen sections (see application references)
Immunoblotting (1 g/ml)
Paraffin sections (2.5 g/ml, pressure cooker pre-treatment required)

Advertencia

Toxicity: Standard Handling (A)

Forma física

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0

Reconstitución

Following initial thaw, aliquot and freeze (-20°C).

Otras notas

To prepare conditioned medium for positive control, incubate HT-1080 cells for 2 h at 37°C in serum-free media containing 100 nM PMA. Collect and centrifuge medium; concentrate as necessary. Does not cross-react with bovine. Will not cross-react with human MMP-2, MMP-3, MMP-8, MMP-9, or MMP-13. For best results with paraffin sections, pre-treat with a pressure cooker; however, heat, saponin or trypsin can be used in place of a pressure cooker. Antibody should be titrated for optimal results in individual systems.
Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol.2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB7, 1434.
Zhang, J., et al. 1993. Clinica Chimica Acta.219, 1.
Woessner, J.F. 1991. FASEB5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1; 99-106.

Información legal

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK Alemania

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Mesenchymal cell transplantation and myocardial remodeling after myocardial infarction.
Dixon, et al.
Circulation, 120, S220-S229 (2018)
Targeted regional injection of biocomposite microspheres alters post-myocardial infarction remodeling and matrix proteolytic pathways.
Dixon, et al.
Circulation, 124, S35-S45 (2018)
Matrix metalloproteinase expression in teeth with apical periodontitis is differentially modulated by the modality of root canal treatment.
Paula-Silva FW, da Silva LA, Kapila YL
Journal of Endodontics null
Iliana Herrera et al.
The Journal of biological chemistry, 288(36), 25964-25975 (2013-08-02)
Idiopathic pulmonary fibrosis is a devastating lung disorder of unknown etiology. Although its pathogenesis is unclear, considerable evidence supports an important role of aberrantly activated alveolar epithelial cells (AECs), which produce a large variety of mediators, including several matrix metalloproteases...
Laura Grau et al.
PloS one, 8(1), e53328-e53328 (2013-01-12)
To identify aggressiveness-associated molecular mechanisms and biomarker candidates in bladder cancer, we performed a SILAC (Stable Isotope Labelling by Amino acids in Cell culture) proteomic analysis comparing an invasive T24 and an aggressive metastatic derived T24T bladder cancer cell line....

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