MABC32-AF488

Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 Antibody, Alexa Fluor^™ 488 Conjugate

from mouse, ALEXA FLUOR^™ 488

• Sinónimos: EBI3-associated protein of 60 kDa, EBIAP, p60, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: ALEXA FLUOR^™ 488
• Clone: monoclonal
• Application: FACS, ICC
• Citations: -

Propiedades

• biological source: mouse
• Quality Segment: 100
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: monoclonal
• species reactivity: mouse, human, rat
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable
• isotype: IgM
• NCBI accession no.: NP_001135770
• UniProt accession no.: Q13501
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... SQSTM1(8878)

Descripción

General description

47.7 kDa calculated.

Sequestosome-1 (UniProt: Q13501; also known as EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62) is encoded by the SQSTM1 (also known as ORCA, OSIL) gene (Gene ID 8878) in human. Sequestosome-1 is a multifunctional protein that binds ubiquitin and regulates the activation of the nuclear factor kappa-B (NF-kappaB) signaling pathway. It functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 (TRAF6) to mediate the activation of NF-kappaB in response to upstream signals. Defects in sequestosome-1 are a cause of Paget disease of bone (PDB), a metabolic bone disease affecting the axial skeleton and is characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Mutations in SQSTM1 gene can lead to frontotemporal dementia and/or amyotrophic lateral sclerosis 3. Frontotemporal dementia is characterized by frontal and temporal lobe atrophy associated with neuronal loss, gliosis, and dementia. Amyotrophic lateral sclerosis is characterized by the death of motor neurons in the brain, brainstem, and spinal cord, resulting in fatal paralysis.

Immunogen

GST-tagged recombinant fragment of 200 amino acids from the internal region of human p62 (Sequestosome-1).

Application

Detect p62 using this mouse monoclonal Anti-p62 (Sequestosome-1) antibody, clone 11C9.2, Alexa Fluor^™ 488 Conjugate, Cat. No. MABC32-AF488, validated for use in Flow Cytometry and Immunocytochemistry.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p62 Sequestosome-1 in A431 and NIH/3T3 cells.

Flow Cytometry Analysis: A 1:400 dilution from a representative lot detected p62 Sequestosome-1 in one million HeLa cells.

Biochem/physiol Actions

Clone 11C9.2, Alexa Fluor^™ 488 Conjugate detects p62 in HeLa cells.

Physical form

Purified mouse monoclonal antibody IgM in PBS with 1.5% BSA and 0.05% sodium azide.

Analysis Note

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected p62 (Sequestosome-1) in HeLa cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• ¿Qué está pasando?: WGK 2