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MABS1925

Sigma-Aldrich

Anti-carboxypeptidase M Antibody, clone 7F9

clone 7F9, from mouse

Sinónimos:

CPM, CP-M

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

7F9, monoclonal

reactividad de especies

rat

envase

antibody small pack of 25 μg

técnicas

immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotipo

IgG2bκ

Nº de acceso NCBI

Nº de acceso UniProt

modificación del objetivo postraduccional

unmodified

Información sobre el gen

rat ... Cpm(314855)

Descripción general

Carboxypeptidase M (UniProt: D4A9Q5; also known as CPM) is encoded by the Cpm gene (Gene ID: 314855) in rat. Rat CPM a glycosylphosphatidylinositol (GPI) anchored metallo-carboxypeptidase that cleaves basic carboxyl-terminal amino acids from various peptides at neutral pH. Rat CPM is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to generate the mature form. The mature CPM displays high specificity and cleaves only C-terminal arginine or lysine residues in a variety of peptide substrates. It is shown to be expressed in the lung and placenta at high levels. Its expression is detected in alveolar cells lacking T1alpha, a type I cell marker protein found in adult lung. Carboxypeptidase M also serves as a macrophage differentiation marker, which is preferentially induced in epithelioid cells of all granuloma types studied, but not in resting macrophages. Hence, it can be employed for detection of minute granuloma even in the presence of non-granulomatous macrophages. Clone 7F9 recognizes CPM expressed in late embryonic rat lung to postnatal day 1. (Ref.: Fujiwara, N., et al. (2007), Respirology 12(1); 54-62; Tsakiris, I., et al. (2012). Lab. Invest. 92(3); 345-361).


Especificidad

Clone 7F9 specifically detects carboxypeptidase M in rat tissues.

Inmunógeno

Epitope: extracellular domain
Membrane fraction of E18.5 embryonic rat lung.

Aplicación

Anti-carboxypeptidase M, clone 7F9, Cat. No. MABS1925, is a mouse monoclonal antibody that detects Carboxypeptidase M and has been tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Research Category
Signaling
Western Blotting Analysis: A representative lot detected carboxypeptidase M in Western Blotting applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).

Immunoprecipitation Analysis: A representative lot immunoprecipitated carboxypeptidase M in Immunoprecipitation applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).

Immunohistochemistry Analysis: A representative lot detected carboxypeptidase M in Immunohistochemistry applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).

Calidad

Evaluated by Immunofluorescence in E19 rat embryo tissue sections.

Immunofluorescence Analysis: A 1:25 dilution of this antibody detected carboxypeptidase M in E19 rat embryo tissue sections.

Descripción de destino

50.32 kDa calculated.

Forma física

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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