PC408

Anti-Green Fluorescent Protein (26-39) Rabbit pAb

liquid, Calbiochem^®

• Sinónimos: Anti-GFP
• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: polyclonal
• Species reactivity: (GFP and some GFP fusions)
• Application: —
• Citations: 3

Propiedades

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: (GFP and some GFP fusions)
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (Immunoblotting (1:2000); Immunocytochemistry (1:200))
• isotype: IgG
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Descripción

General description

Recognizes the ~28 kDa GFP and some GFP fusion proteins.

Anti-Green Fluorescent Protein (26-39), rabbit polyclonal, recognizes the ~28 kDa GFP and some GFP fusion proteins. It is validated for use in Western blotting and immunocytochemistry.

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa GFP and some GFP fusion proteins.

Immunogen

a synthetic peptide corresponding to amino acids 26-39 of Aequorea victoria wild-type green fluorescent protein

Aequora victoria

Application

Immunoblotting (1:2000)

Immunocytochemistry (1:200)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 1 mg/ml BSA.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Cubitt, A.B., et al. 1995. Trends Biochem. Sci.20, 448.

This sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. This antibody has been successfully used in immunoblotting and immunocytochemistry protocols to detect GFP and fusion proteins. It detects a ~28 kDa protein that corresponds to GFP in immunoblots of protein extract from cells transfected with, and overexpressing, GFP isoforms. Antibody should be titrated for optimal results in individual sytems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• ¿Qué está pasando?: WGK 1
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable