ABTS-RO

Roche

ABTS Tablets

formulario

tablet

Nivel de calidad

100

envase

pkg of 20 tablets (11112422001[50mg])
pkg of 20 tablets (11204521001[5mg])

manufacturer/tradename

Roche

color

green

solubilidad

water: soluble

λmax

414 nm

enviado en

wet ice

temp. de almacenamiento

2-8°C

Descripción general

ABTS Tablets are used as substrates in enzyme immunoassays with horseradish peroxidase as a marker enzyme. It is widely used in spectrophotometric methods to measure antioxidant activities of food and beverages.

Aplicación

ABTS Tablets are the ideal substrate for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme.

Características y beneficios

  • ABTS is soluble in aqueous and organic solvents
  • ABTS method is suitable for various pH levels
  • Samples react with ABTS quickly and reach stability within 30 minutes

Envase

  • 11204521001 (5 mg/tablet; each tablet is for 5 mL substrate solution)
  • 11112422001(50 mg/tablet; each tablet is for 50 mL substrate solution)

Nota de preparación

Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of the ABTS buffer solution. This solution has a light-green color. In microtiter plates, it is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.

Nota de análisis

Evaluation: photometric (405nm)

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información legal

ABTS is a trademark of Roche

RIDADR

NONH for all modes of transport

Haralg Seitz and Sarah Schumacher
Molecular Diagnostics null
R Re et al.
Free radical biology & medicine, 26(9-10), 1231-1237 (1999-06-25)
A method for the screening of antioxidant activity is reported as a decolorization assay applicable to both lipophilic and hydrophilic antioxidants, including flavonoids, hydroxycinnamates, carotenoids, and plasma antioxidants. The pre-formed radical monocation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS*+) is generated by oxidation...

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