56575
GenElute™-LPA
Neutral carrier for ethanol precipitation
Sinónimos:
linear polyacrylamide
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 mL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 152,00 € |
Acerca de este artículo
NACRES:
NA.52
UNSPSC Code:
12352200
152,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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storage temp.
2-8°C
General description
Application
GenElute™-LPA has been used to:
- extract DNA from cyanobacterial and algal cultures
- precipitate genomic DNA
- extract and purify DNA from snap-frozen uveal melanoma tissues
- to extract RNA from isolated CD4+ cells
Biochem/physiol Actions
The presence of LPA during ethanol precipitation results in complete recovery of fragments larger than 20 base pairs, whereas most of the DNA is lost if no carrier is used. Very short DNA fragments, less than 20 base pairs, do not coprecipitate with LPA, allowing separation of labeled DNA from unreacted nucleotides by precipitation after the labeling reaction.
GenElute LPA Linear Polyacrylamide is an efficient neutral carrier for precipitating picogram amounts of nucleic acids with ethanol. The nucleic acid precipitate can be collected simply by centrifugation. LPA offers several advantages for recovering DNA or studying DNA-protein interactions, relative to other carriers, such as tRNA or glycogen. tRNA interferes with DNA during phosphorylation with polynucleotide kinase and glycogen competes with protein in DNA-protein interaction studies. In contrast, LPA is completely inert. LPA is synthesized chemically and, therefore, is not contaminated with biological material. The precipitate is visible immediately upon addition of LPA, thus eliminating wait time and low temperature incubation.
Packaging
GenElute LPA is tested for nucleases, and is supplied in nuclease-free water as a 25 mg/ml solution.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Este artículo | |||
|---|---|---|---|
| description Neutral carrier for ethanol precipitation | description sufficient for 70 purifications | description Reagents and consumables for various cell culture sample types. 10, 50 or 250 purifications. | description - |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 15-25°C | storage temp. 2-8°C | storage temp. room temp |
Clase de almacenamiento
10 - Combustible liquids
¿Qué está pasando?
WGK 3
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
EPI (equipos de protección individual)
Eyeshields, Gloves
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