76546

Lipase, immobilized on Immobead 150 from Thermomyces lanuginosus

≥3000 U/g

• Sinónimos: Immobilized lipase, Lipase enzyme
• NACRES: NA.54
• UNSPSC Code: 12352204
• Specific activity: ≥3000 U/g
• Biological source: fungus (Thermomyces lanuginosus)

Propiedades

• biological source: fungus (Thermomyces lanuginosus)
• Quality Segment: 100
• form: powder (or beads)
• specific activity: ≥3000 U/g
• technique(s): analytical sample preparation: suitable
• color: white to off-white
• application(s): life science and biopharma
• storage temp.: 2-8°C

Descripción

General description

Research area: Cell Signaling

Lipase from Thermomyces lanuginosus (TLL) is a basophilic, single-chain protein and thermostable enzyme. It belongs to the alpha/beta-hydrolase fold family. TLL is roughly spherical and is made up of a central seven-stranded β-parallel sheet with five interconnecting α-helices.

Application

Lipase, immobilized on Immobead 150 from Thermomyces lanuginosus has been used:

• as a biocatalyst to study ultrasound-assisted transesterification of a blend of non-edible oils with methanol.
• as a catalyst in the synthesis of medium chain fatty acids (MCFA) and polyunsaturated fatty acids (PUFA) rich oils by enzymatic structuring of flax seed oil.
• for the resolution of chiral compounds and the transesterification production of biodiesel.
• to enhance the efficiency and selectivity of the enzymatic synthesis of ethyl-(R)-2-hydroxy-4-phenylbutyrate, (R)-HPB ester.
• to facilitate a chemoenzymatic process for the continuous synthesis of (S)-7-((tert-Butyldiphenylsilyl)oxy)hept-1-yn-4-ol, a crucial intermediate for Eribulin synthesis, within a packed-bed reactor setup.

Biochem/physiol Actions

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

It participates in catalyzing the hydrolysis of triglycerides at the water-lipid interface and produces mono-, di-glycerides, and free fatty acids. Due to its versatility, this enzyme is applicable in various industries including, detergents, paper, leather, baking, fine chemicals, fuel, and biocatalysts.

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 7.5 and 40°C (tributyrin, Cat. No. 91010, as substrate)

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 3
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• EPI (equipos de protección individual): Eyeshields, Gloves, type N95 (US)