Merck
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83264

Sigma-Aldrich

HEPES buffer solution

1 M in H2O

Sinónimos:
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), HEPES solution
Número de CAS:
Número MDL:
eCl@ss:
32129211
ID de la sustancia en PubChem:
NACRES:
NA.25

formulario

liquid

concentración

1 M in H2O
238 g/L HEPES in H2O

impurezas

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

5.5±0.5

useful pH range

6.8-8.2

densidad

1.07 g/mL at 20 °C

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Descripción general

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicación

HEPES has been used:
  • As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
  • To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
  • As a component of dissection buffer for culturing rat cortical neurons
  • Along with MgCl2, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
  • As a component of Danieu′s aquarium water used in agarose gel preparation

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificado de Análisis

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Certificado de origen

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Más documentos

Quotes and Ordering

Phagocytosis Assay of Microglia for Dead Neurons in Primary Rat Brain Cell Cultures.
Zhao X, et al.
Bio-protocol, 6(8) (2016)
Cultivation of Salmonella enterica serovar Typhimurium in a norepinephrine-containing medium alters in vivo tissue prevalence in swine.
Toscano MJ, et al.
Journal of experimental animal science, 43 , 329-338 (2007)
Novel membrane-based electrochemical sensor for real-time bio-applications.
Alatraktchi FA, et al.
Sensors, 14(11), 22128-22139 (2014)
Group 2 innate lymphoid cells express functional NKp30 receptor inducing type 2 cytokine production.
Salimi M, et al.
Journal of Immunology, 196(1), 45-54 (2016)
"Hypoxia-induced metastasis model in embryonic zebrafish.
Rouhi P, et al.
Nature Protocols, 5(12), 1911-1911 (2010)

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