Merck
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96992

Sigma-Aldrich

Cell Counting Kit - 8

for quantitation of viable cell number in proliferation and cytotoxicity assays

NACRES:
NA.32

Nivel de calidad

100

caducidad

limited shelf life, expiry date on the label

enviado en

ambient

temp. de almacenamiento

2-8°C

Aplicación

Cell Counting Kit-8 (CCK-8) allows convenient assays using WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt), which produces a water-soluble formazan dye upon bioreduction in the presence of an electron carrier, 1-Methoxy PMS. CCK-8 solution is added directly to the cells, no pre-mixing of components is required. WST-8 is bioreduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells. Since the CCK-8 solution is very stable and it has little cytotoxicity, a longer incubation, such as 24 to 48 hours, is possible.
Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS.

pictogramas

Exclamation markHealth hazard

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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